@article{fdi:010094163,
  title = {{G}enomic insights into virulence, biofilm formation, and antimicrobial resistance of multidrug-resistant {H}elicobacter pylori strains of novel sequence types isolated from {V}ietnamese patients with gastric diseases},
  author = {{B}ui, {T}. {T}. and {T}ran, {T}. {T}. {T}. and {N}guyen, {T}. {S}. and {L}e, {T}. {T}. {H}. and {N}guyen, {C}. {L}. and {P}ham, {H}. {N}. and {B}anuls, {A}nne-{L}aure and {L}e, {H}. {S}. and {L}e, {H}. {P}. {A}. and {B}ui, {T}. {T}. and {B}ui, {T}. {S}. and {P}han, {Q}. {H}. and {T}ran, {T}. {H}. {T}. and {N}guyen, {Q}. {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}elicobacter pylori ( {H}. pylori ) is a clinically important pathogen associated with gastric diseases. {H}ere, we characterized the genome of multidrug-resistant {H}. pylori strains of novel sequence types, which were recovered from {V}ietnamese patients with gastritis or a stomach ulcer. {P}henotypic-antibiotic susceptibility testing was performed against amoxicillin, clarithromycin, metronidazol, tetracycline, and levofloxacin using an {E}-test. {T}he whole genome sequence of three {H}. pylori strains was de novo assembled, followed by in silico analysis of multilocus sequence typing ({MLST}), core-genome based phylogeny, genetic determinants associated with virulence, biofilm formation, and antibiotic-resistance. {T}he genome sequence of {H}. pylori strains exhibited a high similarity with the average nucleotide identity ({ANI}) values of 98.5% to 99.2%, carried 5 to 7 pathogenicity islands, and 3 to 6 mobilomes. {T}he {MLST} profile of strains revealed novel sequence types {ST}4511, {ST}4512, and {ST}4513. {C}ore-genome based phylogeny exhibited that the three {H}. pylori strains belong to the {A}sian genotype. {T}hese strains possessed 128 to 131 virulence genes, including toxin-encoding genes cag{A} and vac{A}. {D}ouble amoxicillin-resistant mutations on pbp1 and pbp2, or a mutation on pbp3, triple clarithromycin-resistant mutations on 23{S} r{RNA} gene and a levofloxacin-resistant mutation on gyr{A} were detected in antibiotic-resistant strains. {M}utations on rdx{A} were detected in both metronidazole-resistant and-sensitive strains, whereas frx{A} mutations were detected in only one metronidazole-sensitive strain. {F}inally, a rifamycin-resistant mutation in rpo{B} was also detected. {T}his study provides insights into the genome of multidrug-resistant {H}. pylori strains of a novel sequence type circulating in {V}ietnam for future investigations of its pathobiology and spread through human populations.},
  keywords = {{G}astric disease ; {G}enetic mutations ; {H}elicobacter pylori ; {M}ultidrug-resistance ; {N}ovel sequence type ; {V}irulence gene ; {VIET} {NAM}},
  booktitle = {},
  journal = {{J}ournal of {G}lobal {A}ntimicrobial {R}esistance},
  volume = {43},
  numero = {},
  pages = {237--241},
  ISSN = {2213-7165},
  year = {2025},
  DOI = {10.1016/j.jgar.2025.05.001},
  URL = {https://www.documentation.ird.fr/hor/fdi:010094163},
}