@article{fdi:010093573,
  title = {{E}lucidation of the symbiotic incompatibility mechanisms between {V}igna radiata and {B}radyrhizobium vignae {ORS}3257 mediated by nodulation outer protein {P}2},
  author = {{S}ongwattana, {P}. and {B}oonchuen, {P}. and {P}ruksametanan, {N}. and {T}eamtisong, {K}. and {S}ato, {S}. and {H}ashimoto, {S}. and {H}igashitani, {N}. and {K}awaharada, {Y}. and {A}raragi, {M}. and {O}kazaki, {S}. and {P}iromyou, {P}. and {W}ongdee, {J}. and {G}reetatorn, {T}. and {G}iraud, {E}ric and {B}oonkerd, {N}. and {T}ittabutr, {P}. and {T}eaumroong, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}radyrhizobium vignae {ORS}3257 is an efficient symbiotic strain for {V}igna unguiculata and {V}. mungo but fails with {V}. radiata due to an effector-triggered immunity response mediated by the nodulation outer protein {P}2 ({N}op{P}2). {T}o understand this incompatibility, we identified {N}op{P}2 interacting proteins in {V}. radiata cv. {KPS}1, including enolase, glyceraldehyde-3-phosphate dehydrogenase ({GAPDH}), monodehydroascorbate reductase ({MDHAR}), and serine hydroxymethyltransferase ({SHMT}) as targets. {P}rotein-protein interaction assays confirmed that {N}op{P}2 binds to these enzymes, and further analysis revealed their co-localization on the plasma membrane. {C}omparative transcriptomic analysis revealed {N}op{P}2 stimulates genes related to plant defense response ({PR}1, {PR}5, {MYB}13, and {TAO}1), hydrogen peroxide ({SOD}, {POX}10, and {POX}16), and cell wall lignification ({LAC}). {N}op{P}2 did not alter the expression of genes encoding the target enzymes but interfered with {MDHAR} activity, leading to high {H}2{O}2 accumulation in roots. {T}hese findings suggest that {N}op{P}2 contributes to symbiotic incompatibility in {V}. radiata by inducing a multifaceted defense response and initiating cell wall lignification early in infection.},
  keywords = {},
  booktitle = {},
  journal = {i{S}cience},
  volume = {28},
  numero = {5},
  pages = {112351 [23 p.]},
  year = {2025},
  DOI = {10.1016/j.isci.2025.112351},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093573},
}