@article{fdi:010093447,
  title = {{C}haracterization of size distribution and markers for mosquito extracellular vesicles},
  author = {{R}ey-{C}adilhac, {F}. and {R}achenne, {F}. and {M}arquant, {A}. and {H}im, {J}. {L}. {K}. and {A}ncelin, {A}. and {F}oisor, {V}. and {M}orille, {M}. and {L}yonnais, {S}. and {C}azevieille, {C}. and {M}iss{\'e}, {D}oroth{\'e}e and {P}ompon, {J}ulien},
  editor = {},
  language = {{ENG}},
  abstract = {{E}xtracellular vesicles ({EV}s) are non-replicative, cell-derived membranous structures secreted by potentially all eukaryotic cells, playing a crucial role in intercellular communication. {T}he study of {EV}s requires approaches and tools, which have predominantly been developed for mammalian models. {H}ere, we undertook a multimodal characterization of mosquito {EV}s to provide a technical and knowledge foundation for their study. {F}irst, using a cell line model from {A}edes aegypti and applying multiple analytical technologies (i.e., {NTA}, {TEM}, cryo-{EM}, and {AFM}), we observed that mosquito {EV}s range from 20 to 500 nm in diameter and that a majority are smaller than 100 nm. {S}econd, we showed that smaller {EV}s are secreted in mosquito saliva. {T}hird, we evaluated the capacity of differential centrifugation and size exclusion chromatography to separate mosquito {EV}s, revealing the strengths and weaknesses of each technology. {F}inally, we identified a mosquito homolog of {CD}63 as an extravesicular marker and the mosquito syntenin as a putative luminal marker. {O}verall, our results promote the development of tools and approaches for the study of mosquito {EV}s.},
  keywords = {extracellular vesicles ; mosquito ; microscopy ; protein markers ; syntenin ; tetraspanin ; characterization ; analytical methods},
  booktitle = {},
  journal = {{F}rontiers in {C}ell and {D}evelopmental {B}iology},
  volume = {13},
  numero = {},
  pages = {1497795 [14 p.]},
  ISSN = {2296-634{X}},
  year = {2025},
  DOI = {10.3389/fcell.2025.1497795},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093447},
}