%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Benjeddou, F.
%A Mnif, I.
%A Rossignol, Marie
%A Ghribi, D.
%A Tounsi, S.
%A Chandre, Fabrice
%A Zribi-Zghal, R.
%T Towards sustainable vector control : Innovative substrate identification for developing eco-friendly, cost-effective, and highly potent biopesticides
%D 2025
%L fdi:010093442
%G ENG
%J Biological Control
%@ 1049-9644
%K Bacillus thuringiensis israelensis ; Anophele gambiae ss (kis) ; Bio-sourced insecticide ; Spineless Opuntia ficus-indica cladodes flour ; Highly effective biopesticide
%K TUNISIE
%M ISI:001476195700001
%P 105768 [10 ]
%R 10.1016/j.biocontrol.2025.105768
%U https://www.documentation.ird.fr/hor/fdi:010093442
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-06/010093442.pdf
%V 205
%W Horizon (IRD)
%X The unavailability of biological insecticides for mosquitoes' control in Tunisia and their high cost make chemical pesticides the most used solution. In the present study, the development of a bio-sourced media based on agriculture by-product and free biological material was conducted for the generation of commercially valuable biopesticide from BUPM98 Bacillus thuringiensis israelensis strain. Through the physico-chemical characterization of the spineless Opuntia ficus-indica cladodes flour (SFI flour), an important total organic carbon rate (18.92 %) was detected. Thus, SFI flour was used as a potential carbon source for B. thuringiensis cells growth and deltaendotoxin production. A bio-sourced media based on diluted sea water, SFI flour and soybean meal, was optimized for BUPM98 delta-endotoxin production through the response surface methodology. The adjusted medium improved the production by 58 % compared to the reference medium. Moreover, an additional improvement of 22.85 % in delta-endotoxin synthesis was achieved through cultivating BUPM98 in 1 L shake flasks with baffles under optimal conditions. This enabled the biopesticides production in the novel medium (FPOM-SFI) to reach 2405.5 +/- 45 mg/L. Interestingly, the FPOM-SFI(BUPM98) achieved a more than threefold activity than VectoBac (R) 12AS. In fact, it revealed LC50 values of 0.0039 mg/L and 0.0041 mg/L, against Aedes aegypti and Anophele gambiae larvae, respectively. These findings underscore the substantial potential of the BUPM98 based biopesticide produced in an almost bio-sourced medium for mosquito management in Tunisia as well as in North African countries, as an economical and eco-friendly alternative of chemical insecticides. FPOM-SFI(BUPM98) could be a sustainable and affordable pest control solution.
%$ 052