@article{fdi:010093442,
  title = {{T}owards sustainable vector control : {I}nnovative substrate identification for developing eco-friendly, cost-effective, and highly potent biopesticides},
  author = {{B}enjeddou, {F}. and {M}nif, {I}. and {R}ossignol, {M}arie and {G}hribi, {D}. and {T}ounsi, {S}. and {C}handre, {F}abrice and {Z}ribi-{Z}ghal, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he unavailability of biological insecticides for mosquitoes' control in {T}unisia and their high cost make chemical pesticides the most used solution. {I}n the present study, the development of a bio-sourced media based on agriculture by-product and free biological material was conducted for the generation of commercially valuable biopesticide from {BUPM}98 {B}acillus thuringiensis israelensis strain. {T}hrough the physico-chemical characterization of the spineless {O}puntia ficus-indica cladodes flour ({SFI} flour), an important total organic carbon rate (18.92 %) was detected. {T}hus, {SFI} flour was used as a potential carbon source for {B}. thuringiensis cells growth and deltaendotoxin production. {A} bio-sourced media based on diluted sea water, {SFI} flour and soybean meal, was optimized for {BUPM}98 delta-endotoxin production through the response surface methodology. {T}he adjusted medium improved the production by 58 % compared to the reference medium. {M}oreover, an additional improvement of 22.85 % in delta-endotoxin synthesis was achieved through cultivating {BUPM}98 in 1 {L} shake flasks with baffles under optimal conditions. {T}his enabled the biopesticides production in the novel medium ({FPOM}-{SFI}) to reach 2405.5 +/- 45 mg/{L}. {I}nterestingly, the {FPOM}-{SFI}({BUPM}98) achieved a more than threefold activity than {V}ecto{B}ac ({R}) 12{AS}. {I}n fact, it revealed {LC}50 values of 0.0039 mg/{L} and 0.0041 mg/{L}, against {A}edes aegypti and {A}nophele gambiae larvae, respectively. {T}hese findings underscore the substantial potential of the {BUPM}98 based biopesticide produced in an almost bio-sourced medium for mosquito management in {T}unisia as well as in {N}orth {A}frican countries, as an economical and eco-friendly alternative of chemical insecticides. {FPOM}-{SFI}({BUPM}98) could be a sustainable and affordable pest control solution.},
  keywords = {{B}acillus thuringiensis israelensis ; {A}nophele gambiae ss (kis) ; {B}io-sourced insecticide ; {S}pineless {O}puntia ficus-indica cladodes flour ; {H}ighly effective biopesticide ; {TUNISIE}},
  booktitle = {},
  journal = {{B}iological {C}ontrol},
  volume = {205},
  numero = {},
  pages = {105768 [10 p.]},
  ISSN = {1049-9644},
  year = {2025},
  DOI = {10.1016/j.biocontrol.2025.105768},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093442},
}