@article{fdi:010093434,
  title = {{C}ombined immuno{CAP} and western blot for the diagnosis of {A}spergillus lung disease},
  author = {{P}etit, {E}. and {C}ortaredona, {S}{\'e}bastien and {S}icco, {S}. and {R}anque, {S}. and {M}enu, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}ulmonary aspergillosis is a major global health concern, yet its diagnosis remains challenging. {A}spergillus-specific {I}g{G} measurement is essential for identifying chronic and allergic forms. {O}bjective: {T}his study aimed to evaluate a quantitative method, the {I}mmuno{CAP} assay {I}g{G} m3 ({ICAP}) ({P}hadia-{T}hermo{F}isher {S}cientific, {W}altham, {USA}), a qualitative method, the {A}spergillus {I}g{G} {W}estern blot kit ({A}sp-{WB}) ({LDB}io {D}iagnostics, {L}yon, {F}rance) and a combination of both methods for the diagnosis of {A}spergillus lung disease. {M}ethods: {A} retrospective study was conducted at the {U}niversity {H}ospital of {M}arseille, {F}rance, during 1 year. {P}atients undergoing {A}spergillus serology were divided into three groups: {G}roup 1 ({G}1) with {ICAP} >= 40 mg{A}/{L} and positive {A}sp-{WB}, {G}roup 2 ({G}2) with {ICAP} >= 40 mg{A}/{L} and negative {A}sp-{WB} and {G}roup 3 ({G}3) with {ICAP} < 40 mg{A}/{L} and positive {A}sp-{WB}. {D}ata were collected on demographics, underlying diseases, imaging and biological outcomes. {P}atients were classified according to their {A}spergillus lung disease, whether acute pulmonary aspergillosis, chronic pulmonary aspergillosis ({CPA}), allergic broncho-pulmonary aspergillosis ({ABPA}), colonisation or {A}spergillus sensitisation. {R}esults: {A} total of 536 patients were studied: 173 in {G}1, 204 in {G}2 and 200 in {G}3, with 38 patients found in several groups. {T}he primary underlying disease was cystic fibrosis in 44.6% of patients. {T}wenty-two patients were diagnosed with {ABPA}. {T}he number of diagnosed {ABPA} cases in {G}1 (20; 11.6%) combining positive {ICAP} and {A}sp-{WB} was significantly higher than that found in the groups with a single positive test result (p < 0.001). {F}ifteen patients were diagnosed with {CPA}. {I}solated positive {W}estern blot ({G}3) identified five cases of aspergilloma. {S}ignificantly fewer {A}spergillus lung diseases were diagnosed in isolated positive {ICAP} {G}2 (8.8%) than in {G}1 (53.8%) and {G}3 (42.5%) (p < 0.001). {C}onclusions: {T}his study highlights the benefits of combining {A}sp-{WB} and {ICAP} for the diagnosis of {A}spergillus lung disease and the relatively high false-positive rate in patients with isolated positive {ICAP} results.},
  keywords = {{ABPA} ; aspergillus lung disease ; aspergillus sensitisation ; {CPA} ; diagnostic tests ; pulmonary aspergillosis ; serology ; {FRANCE}},
  booktitle = {},
  journal = {{M}ycoses},
  volume = {68},
  numero = {4},
  pages = {e70058 [9 p.]},
  ISSN = {0933-7407},
  year = {2025},
  DOI = {10.1111/myc.70058},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093434},
}