@article{fdi:010093413,
  title = {{R}evealing the role of the {AGO}4 gene against rice hoja blanca virus : from transformation to protein structure},
  author = {Ñañez, {J}. and {V}aldes, {S}. and {G}allego, {M}. {C}. and {R}ebolledo, {M}. {C}. and {L}orieux, {M}athias and {A}lvarez, {M}. {F}. and {C}havarriaga, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice hoja blanca virus ({RHBV}), transmitted by the insect vector {T}agosodes orizicolus, poses a significant threat to rice cultivation. {H}ere, we use {CRISPR}/{C}as9 technology to produce specific mutations in the {AGO}4 gene of {O}ryza sativa, using the {F}edearroz 2000 variety, with the aim of elucidating the participation of the gene in resistance to {RHBV}. {W}e obtained 14 edited plants that presented with deletions of one, two, and three nucleotides in the sequence of exon 23 of the {AGO}4 gene. {P}henotypic evaluations showed an increase in susceptibility to {RHBV} in the edited lines. {W}e identified the presence of {RHBV} in the leaf tissue of infected plants by amplifying the nucleoprotein, {NS}3, and {NS}4 genes of the virus. {U}sing {RT}-q{PCR}, we analyzed the expression patterns of the {AGO}4 gene, showing that in the edited lines, the expression profiles are similar to the susceptible control. {F}urthermore, modeling of the tertiary structure of the {AGO}4 protein and its mutant variant demonstrated changes in the {PIWI} domain and the presence of the {DDH} catalytic triad, confirming its role in mediating resistance to {RHBV}. {O}ur study reveals the functional importance of the rice {AGO}4 gene in {RHBV} resistance.},
  keywords = {{O}ryza sativa ; {T}enuivirus ; {A}lphafold2 ; argonaute protein ; {CRISPR}/{C}as9},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {16},
  numero = {},
  pages = {1517321 [11 p.]},
  ISSN = {1664-462{X}},
  year = {2025},
  DOI = {10.3389/fpls.2025.1517321},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093413},
}