@article{fdi:010093289,
  title = {{L}ife cycle of the root-knot nematode {M}eloidogyne javanica in papaya and histological analyses of root infection and gall development},
  author = {{C}oulibaly, {K}.{R}.{L}. and {T}hio, {B}. and {C}ollin, {M}yriam and {K}oita, {K}. and {S}anou, {J}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{P}apaya ({C}arica papaya {L}.) is susceptible to attacks by root-knot nematodes ({RKN}), which lead to significant production losses. {U}nderstanding the life cycle of {RKN} in papaya is essential for developing effective control strategies and screening for natural resistance in papaya cultivars. {I}n this study, the development of the {RKN} {M}eloidogyne javanica was assessed in the susceptible papaya variety {S}olo8 over a period of 35 days, using microscopic observation of inoculated roots stained with fuchsin. {B}y 9 days after inoculation ({DAI}), second-stage juveniles ({J}2s) were observed migrating through the root cortex and feeding within the vascular cylinder. {A}t 16 {DAI}, galls containing enlarged {J}2s had formed within the stele, where feeding sites were established. {Y}oung females began appearing within the galls at 26 {DAI}, and mature pear-shaped females with egg masses were present by 35 {DAI}. {R}emarkably, some egg masses were deposited within the cortex, where a new generation of {J}2s hatched inside the root. {H}istological cross-sections of galls revealed that feeding sites initiated around the nematode head within the stele by 9 {DAI}, with four to eight multinucleated giant cells ({GC}s) present at these sites. {T}hese {GC}s, located within xylem and protophloem elements, were progressively encased by additional cell layers from adjacent vascular and cortical tissues as the nematode developed within the gall at 16, 26, and 35 {DAI}. {T}he {GC}s reached maximum size by 16 {DAI}. {C}ytoplasmic analysis showed that {GC}s were rich in proteins, as evidenced by {N}aphtol {B}lue {B}lack staining, and that their cell walls were strongly stained for polysaccharides using {PAS}. {T}his study offers comprehensive histological insights into nematode development within papaya roots, underscoring that screening papaya genotypes for {RKN} resistance should consider egg mass production within the root tissue.},
  keywords = {{BURKINA} {FASO} ; {FRANCE} ; {BOBO} {DIOULASSO} ; {MONTPELLIER}},
  booktitle = {},
  journal = {{T}ropical {P}lant {P}athology},
  volume = {50},
  numero = {1},
  pages = {30 [11 ]},
  ISSN = {1983-2052},
  year = {2025},
  DOI = {10.1007/s40858-025-00723-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093289},
}