@article{fdi:010093061,
  title = {{B}iofilm formation and whole genome analysis of {MDR} {K}lebsiella {P}neumoniae strains isolated from hospital acquired infections in tertiary hospitals in {D}akar, {S}enegal},
  author = {{N}diaye, {I}. and {S}ow, {O}. and {C}iss{\'e}, {A}. and {S}ambe {B}a, {B}. and {T}hiam, {F}. and {B}oye, {M}.{M}. and {D}i{\`e}ye, {B}. and {F}all, {C}. and {D}ieye, {Y}. and {D}ieng, {A}. and {D}iop, {A}. and {C}onstantin de {M}agny, {G}uillaume and {S}eck, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{K}lebsiella pneumoniae is widely recognized as an opportunistic pathogen in both hospital and community settings. {I}t is a key member of the {ESKAPE} group, which comprises priority microorganisms of major concern owing to their antibiotic resistance. {T}he resistance of {K}. pneumoniae, particularly related to {E}xtended-{S}pectrum ?-{L}actamases ({ESBL}s), poses a significant global public health challenge. {T}he combination of its {M}ultidrug {R}esistance ({MDR}) phenotype and various pathogenicity factors increases its potential to cause severe clinical infections. {B}iofilm formation was assessed via a semiquantitative microtiter technique. {W}e employed various bioinformatics tools to analyze the {A}ntimicrobial {R}esistance ({AMR}), virulence factors, plasmid replicons, and genomic diversity of the {CRKP} isolates. {O}verall, among the 24 {K}. pneumoniae isolates, most produced strong biofilms (n = 21), with some exhibiting moderate (n = 1) or weak (n = 2) biofilm production. {A}n alarming level of resistance to multiple classes of antibiotics was correlated with the presence of various resistance genes, including those for beta-lactams (bla{OXA}-48, bla{OXA}-181, bla{CTX}-{M}15, bla{TEM} and bla{SHV}), aminoglycosides (aph(6)-{I}d, aac(3)-{II}e, aad{A}2, ant(3')-{II}a, aph(3')-{I}a and aac(6')-{I}b-cr), and quinolones (qnr{A}, qnr{B}, qnr{S}, {CRP}, and emr{R}). {V}arious efflux pumps, such as {K}pn{GH}, oqx{AB}, acr{AB}, acr{D}, and {K}pn{EF}, are ubiquitously distributed across {MDR} {K}. pneumoniae strains. {S}everal virulence-associated genes encoding type 1 fimbriae (fim{H}), type 3 fimbriae (mrk{A}), efflux pumps (acr{AB}, oqx{AB}), enterobactin (ent{A}, ent{B}, fep{C}), and yersiniabactin (irp1, irp2, ytb{A}, ybt{E}, ybt{P}, ybt{Q}, ytb{T}, ytb{U}, ytb{X}) have been identified. {G}enetically, the isolates presented high diversity, with 18 {S}equence {T}ypes ({ST}s) and an average of 70.1% accessory genes. {O}n the basis of {SNP} distance and pairwise {ANI} analysis, the majority of {K}. pneumoniae isolates were grouped into one clade. {T}he high plasticity of {K}. pneumoniae in the acquisition of an {MDR} phenotype, combined with the phenotypic and genotypic factors described in this report, underscores the challenges in achieving effective clinical therapy with the available antibiotics. {T}he findings also emphasize the critical need for the surveillance of multidrug-resistant pathogens in clinical settings in {S}enegal, as well as the need to evaluate their prevalence, propagation, and impact on patient health outcomes.},
  keywords = {{SENEGAL} ; {DAKAR}},
  booktitle = {},
  journal = {{J}ournal of {B}acteriology and {M}ycology},
  volume = {11},
  numero = {2},
  pages = {1222 [9 ]},
  ISSN = {2471-0172},
  year = {2024},
  URL = {https://www.documentation.ird.fr/hor/fdi:010093061},
}