@article{fdi:010092918,
  title = {{A} novel seminested polymerase chain reaction assay with high sensitivity and robust specificity enables simultaneous detection of human and animal bocaviruses},
  author = {{P}iewbang, {C}. and {Z}ahro, {A}. {N}. and {P}oonsin, {P}. and {P}uenpa, {J}. and {P}hupolphan, {C}. and {K}osoltanapiwat, {N}. and {N}gamprasertwong, {T}. and {C}laude, {J}ulien and {L}ekchareonsuk, {P}. and {P}oovorawan, {Y}. and {T}echangamsuwan, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {B}ocaviruses ({B}o{V}s), belonging to the {P}arvoviridae family, pose significant challenges in detection due to their genetic diversity and cross-species transmission capabilities. {E}fficient and broad-spectrum detection methods are essential for understanding {B}o{V} epidemiology and addressing potential zoonotic risks. {M}ethods {W}e developed a seminested polymerase chain reaction ({PCR}) assay for simultaneous detection of diverse {B}o{V} species across human and animal hosts. {P}rimers were designed by analyzing 765 {B}o{V} genome sequences, targeting conserved regions spanning the {NP}1 to {VP}2 genes. {S}ensitivity was determined through analytical tests, and specificity was evaluated against 39 non-{B}o{V} viruses. {V}alidation was performed using spiked biological samples, and the method was applied to 552 clinical samples from 542 hosts, encompassing a broad range of mammalian species.{R}esults {T}he assay demonstrated high sensitivity, detecting {B}o{V}s at concentrations as low as 0.2 copies/mu {L}. {S}pecificity tests confirmed no cross-reactivity with other viral families. {V}alidation using 37 strains representing 29 {B}o{V} species affirmed its broad efficacy. {B}o{V}s were identified across diverse hosts, including humans, bats, canines, porcines, rodents, and felines. {A}dditionally, novel host associations were observed, such as {P}anthera uncia bocaparvovirus ({P}u{B}o{V}) in a tiger and serval cat, canine bocavirus 2 ({CB}o{V}-2) in raccoon dogs, and feline bocaviruses ({FB}o{V}) in murid rodents. {H}uman bocaviruses were also detected in monkey samples, indicating potential pathogen spillover.{C}onclusions {T}his seminested {PCR} method provides a sensitive and specific tool for {B}o{V} detection, enhancing surveillance in human and animal populations. {I}t is instrumental in monitoring zoonotic risks and emerging infectious threats, offering critical insights into {B}o{V} epidemiology and cross-species transmission dynamics. {A} seminested pan-{PCR} assay enables broad-range bocavirus detection across human and animal hosts with high sensitivity and specificity. {T}his method enhances viral surveillance, facilitates zoonotic risk assessment, and supports the {O}ne {H}ealth approach in monitoring emerging bocavirus infections and cross-species transmission.},
  keywords = {bocavirus ; cross-species transmission ; molecular monitoring ; {O}ne {H}ealth ; seminested pan-{PCR}},
  booktitle = {},
  journal = {{J}ournal of {I}nfectious {D}iseases},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[11 p.]},
  ISSN = {0022-1899},
  year = {2025},
  DOI = {10.1093/infdis/jiaf090},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092918},
}