@article{fdi:010092872,
  title = {{RUNCOV} : a one-pot triplex real-time {RT}-{LAMP} as a point-of-care diagnostic tool for detecting {SARS}-{C}o{V}-2},
  author = {{R}ob{\`e}ne, {I}. and {J}ouen, {E}. and {M}aillot-{L}ebon, {V}. and {F}enelon, {B}. {T}. and {H}ascoat, {J}. and {P}ecrix, {Y}. and {R}ichard, {D}amien and {J}affar-{B}andjee, {M}. {C}. and {M}avingui, {P}. and {C}hiroleu, {F}. and {B}ecker, {N}. and {P}oubeau, {P}. and {R}amiandrisoa, {M}. and {S}in, {M}. and {C}ostet, {L}. and {L}aurent, {A}. and {L}aurent, {P}. and {C}habirand, {A}. and {M}oreau, {A}. and {R}eynaud, {B}. and {J}euffrault, {E}. and {C}etre-{S}ossah, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}iven the risk of zoonotic disease emergence, including new {SARS}-{C}o{V}-2 variants of {COVID}-19, rapid diagnostic tools are urgently needed to improve the control of the spread of infectious diseases. {A} one-pot triplex real-time {RT}-{LAMP} (reverse-transcription-loop-mediated isothermal amplification) assay, based on two regions of the genome {SARS}-{C}o{V}-2-specifically the {O}rf1ab and {N} genes-along with one internal control, the human {RN}ase {P} gene, was developed. {T}he multiplexing relies on the distinct melting peaks produced during an annealing step. {T}his tool, named {RUNCOV}, was compared to the gold-standard reverse-transcription real-time quantitative {PCR} ({RT}-q{PCR}) assay. {A} simple sample preparation step was designed alongside the assay, making it ready for use on site, as a point-of-care diagnostic tool. {RUNCOV} is rapid (typically less than 40 minutes), highly sensitive and specific. {W}hen tested on clinical samples with known {SARS}-{C}o{V}-2 status, its limit of detection ({LOD}) ranges between 5 and 20 copies per reaction and its diagnostic sensitivity (97.44%) and specificity (100%) values are high compared to the {RT}-q{PCR} gold standard. {T}hese results were supported with an extensive in silico analysis of over 14 million genomes, demonstrating this tool was capable of detecting all known {SARS}-{C}o{V}-2 variants, including the most recent ones {KP}.3.1.1 and {BA}2.86.1. {T}his molecular assay is portable, as demonstrated when it was used successfully in {L}a {R}{\'e}union in different contexts outside the laboratory.},
  keywords = {{SARS}-{C}o{V}-2 ; triplex-{RT}-{LAMP} ; detection ; point-of-care testing},
  booktitle = {},
  journal = {{B}iology {M}ethods and {P}rotocols},
  volume = {10},
  numero = {1},
  pages = {bpaf010 [16 p.]},
  year = {2025},
  DOI = {10.1093/biomethods/bpaf010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092872},
}