@article{fdi:010092868,
  title = {{A}ssessment of {MALDI}-{TOF} {MS} for the identification of cultural heritage insect pests},
  author = {{H}amadouche, {D}. and {D}iarra, {A}dama {Z}an and {F}ohrer, {F}. and {B}{\'e}renger, {J}. {M}. and {B}enakhla, {A}. and {A}lmeras, {L}. and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}hen heritage properties and contents are threatened by various harmful insects, especially coleopterans, rapid and reliable pest identification is required for effective management. {W}hile traditional methods have limitations, {MALDI}-{TOF} {MS} has recently emerged as a groundbreaking identification tool. {I}n this study, our aim was to assess the effectiveness of {MALDI}-{TOF} {MS} in identifying cultural heritage insect pests belonging to the {C}oleoptera order. {T}o do so, adult, larva, and exuviae from eight species of {C}oleoptera ({L}asioderma serricorne (n = 92), {A}ttagenus smirnovi (n = 87), {T}rogoderma versicolor (n = 97), {R}eesa vespulae (n = 74), {A}nthrenus verbasci (n = 68), {A}nthrenus pimpinellae (n = 34), {A}nthrenus flavipes (n = 30), and {T}hylodrias contractus (n = 19), were subjected to {MALDI}-{TOF} {MS} after optimisation of the sample preparation parameters. {T}his optimisation was based on three key parameters influencing spectrum quality: the choice of body part, the buffer volume for protein extraction, and the method and duration of sample homogenisation. {R}eproducible intra-species {MS} spectra for each developmental stage were obtained. {T}he species specificity of {MS} spectra for each developmental stage supported the use of {MALDI}- {TOF} {MS} for specimen classification. {S}pectra from 68 samples, including four specimens per species and stage, were added to our homemade reference arthropod {MS} database ({DB}). {A}mong spectra classified as being consistent, 97.10% (335/345) of the samples were correctly classified with a relevant identification score. {T}he morphological identification of all adult specimens in the reference {MS} spectra {DB} was confirmed by molecular biology, and the sequences were deposited in {G}en{B}ank. {T}his study is the first demonstration of the effective application of {MALDI}-{TOF} {MS} as a reliable tool for identifying various developmental stages of {C}oleoptera pests which pose a threat to the cultural heritage of {F}rance.},
  keywords = {{C}oleopteran ; {H}eritage ; {MALDI}-{TOF} {MS} ; {E}ntomology ; {P}est ; {FRANCE}},
  booktitle = {},
  journal = {{I}nternational {B}iodeterioration and {B}iodegradation},
  volume = {199},
  numero = {},
  pages = {106033 [11 ]},
  ISSN = {0964-8305},
  year = {2025},
  DOI = {10.1016/j.ibiod.2025.106033},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092868},
}