@article{fdi:010092867,
  title = {{E}valuation of the {A}n{T}at {A}/{B} and {L}i{T}at {A}/{B} primers for the detection of {T}rypanosoma brucei gambiense},
  author = {{I}lboudo, {H}. and {N}'{D}jetchi, {M}. {K}. and {K}abor{\'e}, {W}. {J}. and {K}abor{\'e}, {J}. and {T}raor{\'e}, {B}. {M}. and {T}ahita, {M}. {C}. and {A}houty, {B}. {A}. and {D}eborggraeve, {S}. and {E}loiflin, {R}. and {S}egard, {A}deline and {B}ucheton, {B}runo and {K}offi, {M}. and {J}amonneau, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{E}limination of gambiense human {A}frican trypanosomiasis (g{HAT}) as a public health problem has been reached or is in sight in a number of endemic foci and the next step is now to reach the elimination of transmission. {T}he ability to detect {T}rypanosoma brucei gambiense ({T}. b. gambiense) in both the last human cases and in a suspected animal reservoir becomes increasingly important to reach this goal. {W}e have evaluated here the diagnostic performance of the {A}n{T}at {A}/{B} and {L}i{T}at {A}/{B} primers in comparison with the {TBR}, {T}gs{GP} and nested {T}gs{GP} {PCR}s that are currently used for the molecular diagnosis of g{HAT}. {T}he evaluation was based on serial {DNA} dilutions from two {T}. b. gambiense strains for sensitivity, purified reference strains for specificity and field strains isolated from pigs in {C}ote d'{I}voire for field application. {R}esults showed that the two {PCR}s ({A}n{T}at {A}/{B} and {L}i{T}at {A}/{B}) are not specific for {T}. b. gambiense, limiting their relevance for studies on suspected animal reservoirs. {H}owever, they could represent complementary tools to improve the molecular diagnosis of g{HAT} in the elimination process even if the detection limit was lowest than for the {T}gs{GP} {PCR}. {T}he results also once more suggest that nested {T}gs{GP} {PCR} should be interpreted with caution as they may lead to an over-estimation of the {T}. b. gambiense prevalence particularly in animal studies.},
  keywords = {{H}uman {A}frican trypanosomiasis ; {M}olecular diagnosis ; {PCR} ; {T}rypanosoma brucei gambiense ; {A}nimal reservoir ; {E}limination ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{E}xperimental {P}arasitology},
  volume = {271},
  numero = {},
  pages = {108929 [6 p.]},
  ISSN = {0014-4894},
  year = {2025},
  DOI = {10.1016/j.exppara.2025.108929},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092867},
}