@article{fdi:010092862,
  title = {{H}igh correlation between detection of dengue {I}g{G} from dried blood spots and serum using an indirect {I}g{G} {ELISA} assay : a validation study in {F}ortaleza, {B}razil},
  author = {{Z}ahreddine, {M}. and {P}arra, {B}. and {P}ierce, {L}. and {F}erreira de {O}liveira, {D}. and {C}arabali, {M}. and {C}harland, {K}. and {A}breu, {K}. and {R}idde, {V}al{\'e}ry and {L}ima, {D}. {M}. and {Z}inszer, {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {D}engue virus ({DENV}) seroprevalence studies often rely on {E}nzyme-{L}inked {I}mmunosorbent {A}ssay ({ELISA}) testing of serum samples, but {ELISA} testing of dried blood spot ({DBS}) samples offer several advantages for field-based research in resource-limited settings. {H}owever, {DBS}' limited sample volume can be challenging for test sensitivity, requiring validation studies with standard methods (e.g., analysis of serum through {ELISA}s or {P}laque {R}eduction {N}eutralization {T}ests ({PRNT}s)). {I}n preparation for a large cluster randomized controlled trial, we conducted a pilot study in 2019 to validate the use of {DBS} compared to serum samples for {DENV} {I}g{G} testing. {W}e aimed to identify the optimal {DBS} dilution for {I}g{G} detection and to estimate the correlation, magnitude of agreement, and sensitivity and specificity of {I}g{G} detection in {DBS} versus serum samples. {M}ethodology/{P}rincipal {F}indings {W}e conducted this pilot validation study among 119 healthy participants in {F}ortaleza, {B}razil to evaluate and optimize the detection of {DENV} {I}g{G} from {DBS} compared to serum. {E}ach participant provided paired {DBS} and venous blood samples, which were evaluated for {DENV} {I}g{G} using the {P}anbio {D}engue {I}g{G} indirect {ELISA}. {DBS} elution diluted 1:4 was optimal compared with serum results, with high correlation (r= 0.98) and near-perfect agreement (kappa = 0.95). {A}t this dilution, {DBS} had a sensitivity of 100%, a specificity of 92.3%, a 97.9% positive predictive value, and a 100% negative predictive value compared with serum. {C}onclusions/{S}ignificance {T}hese results validate using {DBS} instead of serum for detection of prior dengue infection among similar populations in endemic regions, without sacrificing test sensitivity and specificity. {T}he validity of using {DBS} for {ELISA} to detect prior dengue infection could have important implications for field-based research. {A} limitation to this study was that the potential for misclassification due to cross-reactivity (e.g., with {Z}ika virus, {Y}ellow {F}ever vaccine) was not assessed.},
  keywords = {{BRESIL}},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {19},
  numero = {2},
  pages = {e0012880 [14 p.]},
  ISSN = {1935-2735},
  year = {2025},
  DOI = {10.1371/journal.pntd.0012880},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092862},
}