@article{fdi:010092858,
  title = {{T}wo lateral organ boundary domain transcription factors {H}v{CRL}1 and {H}v{CRL}1{L}1 regulate shoot-borne root formation in barley ({H}ordeum vulgare {L}.)},
  author = {{N}guyen, {T}. {D}. and {G}onin, {M}. and {M}otyka, {M}. and {C}hampion, {A}ntony and {H}ensel, {G}. and {G}antet, {P}ascal and {B}ergougnoux, {V}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lant-specific {LOB}-domain ({LBD}) transcription factors are crucial in post-embryonic root initiation. {I}n cereals, the fibrous root system comprises primary and seminal roots that develop during embryogenesis and lateral- and crown roots that develop post-embryonically from root or stem, respectively. {I}n rice, the {CROWNROOTLESS}1 ({CRL}1) {LBD} transcription factor is the core regulator of crown root initiation and a direct target of the auxin response factor ({ARF})-mediated auxin signaling pathway. {O}rthologs of {CRL}1 have been identified and characterized in several species, where their role in crown root initiation has been validated. {I}n barley, we identified two genes phylogenetically closely related to the rice {CRL}1 genes that we named {H}v{CRL}1 and {H}v{CRL}1{L}1. {U}sing a crown root inducible system ({CRIS}), we identified that both {H}v{CRL}1 and {H}v{CRL}1{L}1 are expressed in response to auxin during the early steps of crown root differentiation in stem base, with {H}v{CRL}1 transcripts being accumulated quickly during the first hour of treatment. {T}ransient activation assays in rice protoplast showed that {H}v{CRL}1 could bind the {LBD}-box, a consensus {DNA} sequence recognized by {LBD} transcription factors, whereas {H}v{CRL}1{L}1 did not. {B}oth genes can partially complement the crl1 rice mutant. {L}oss-of-function mutation in each gene drastically impairs crown root formation in barley. {T}hese data show that {H}v{CRL}1 and {H}v{CRL}1{L}1 are both involved in the regulation of crown root formation in barley but that these two transcription factors likely act through distinct and complementary pathways in this developmental process.},
  keywords = {{C}rown root formation ; {LBD} transcription factor ; {C}ereal ; {B}arley ; {R}ice},
  booktitle = {},
  journal = {{J}ournal of {P}lant {G}rowth {R}egulation},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[13 p.]},
  ISSN = {0721-7595},
  year = {2025},
  DOI = {10.1007/s00344-025-11667-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092858},
}