@article{fdi:010092800,
  title = {{T}he {A}pplication of {C}ulturomics to {E}xplore {A}frican {S}kin {M}icrobiota},
  author = {{N}diaye, {C}. and {B}assene, {H}. and {F}onkou, {M}. {D}. {M}. and {F}enollar, {F}. and {L}agier, {J}. {C}. and {R}aoult, {D}. and {S}okhna, {C}heikh},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ver the past 12 years, culturomics, a high-throughput culture method, has been developed, considerably widening the repertoire of known cultured bacteria. {A}n exhaustive database, including a list of microbes isolated by culture from human skin, was recently established by performing a review of the literature. {T}he aim of the present study was to use the culturomics approach to explore the {A}frican skin microbiota. {S}kin swabs from the palms of human hands were collected between {J}anuary and {D}ecember 2016 from healthy subjects from the villages of {D}ielmo and {N}diop in rural {S}enegal. {T}hree culture media were selected for the isolation of bacteria in aerobic conditions. {B}acterial colonies were subjected to matrix-assisted laser desorption ionization-time of flight mass spectroscopy and the 16{S} r{RNA} gene was sequenced for unidentified colonies. {A} total of 176 bacterial species were isolated. {T}his increased the repertoire of bacterial species on the skin by 14.0%, by adding 71 bacteria, including seven new species. {T}he culturomics approach characterizing microbial diversity has significantly changed our view of the skin microbiota, raising many important questions about the host-microorganism relationship and its relevance to skin diseases. {I}n particular, the difference between the palm microbiota of these {A}frican populations (composed mainly of the genera {S}taphylococcus, {A}rthrobacter, {B}acillus, and {M}icrobacterium) and that of {W}estern populations, whose main genera are {S}taphylococcus, {P}ropionibacterium, {M}icrococcus, {C}orynebacterium, {E}nhydrobacter, and {S}treptococcus. {T}his study demonstrates the need to continue to explore the skin microbiome using the culturomics approach},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {111},
  numero = {6},
  pages = {1331--1337},
  ISSN = {0002-9637},
  year = {2024},
  DOI = {10.4269/ajtmh.23-0165},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092800},
}