@article{fdi:010092726,
  title = {{I}solation and characterization of {A}cinetobacter phage v{A}ba{IN}10 active against carbapenem-resistant {A}cinetobacter baumannii ({CRAB}) isolates from healthcare-associated infections in {D}akar, {S}enegal},
  author = {{N}diaye, {I}. and {D}ebarbieux, {L}. and {S}ow, {O}. and {B}a, {B}. {S}. and {D}iagne, {M}. {M}. and {C}iss{\'e}, {A}. and {F}all, {C}. and {D}ieye, {Y}. and {D}ia, {N}. and {C}onstantin de {M}agny, {G}uillaume and {S}eck, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}arbapenem-resistant {A}cinetobacter baumannii ({CRAB}) is a critical antimicrobial resistance threat and a {WHO}-prioritized pathogen. {W}ith intrinsic resistance to multiple antibiotics and the emergence of pan-resistant isolates, {CRAB} infections are challenging to treat, often relying on polymyxins, tigecycline, aminoglycosides, or combinations, though co-resistance is rising globally. {P}hage therapy is considered as a potential treatment for multidrug-resistant {A}. baumannii . {T}his study focused on isolating and characterizing phages active against {CRAB} strains from healthcare-associated infections in {D}akar, {S}enegal. {M}ethods: {A} lytic phage, {A}cinetobacter v{A}ba{IN}10, was isolated from wastewater collected at the {A}ristide {L}e {D}antec {H}ospital in {D}akar, {S}enegal. {I}solation, host range, efficiency of plating, temperature and p{H} stability, lysis kinetics, one-step growth test, sequencing, and genomic analysis were performed. {R}esults: {P}hage v{A}ba{IN}10 belongs to the class {C}audoviricetes and the genus {F}riunavirus . {I}ts genome is 40,279 bp in size. {P}hage v{A}ba{IN}10 is stable across a wide p{H} range (3-9) and temperature range (25 degrees {C}- 60 degrees {C}). {T}he phage's lytic activity was evaluated at different multiplicities of infection ({MOI}): {MOI} 10, 1, and 10-1. {A}ll {MOI}s significantly reduced the growth of host bacteria. {T}he one-step growth curve showed that v{A}ba{IN}10 had a latency period of 25 min and a burst size of approximately 4.78 x 10 3 phages per infected bacterial cell. {N}o t{RNA}, mt{RNA}, clustered regularly interspaced short palindromic repeat, virulence factors, or antibiotic resistance genes were found in the genome. {C}onclusions: {T}he biological and genomic characteristics of v{A}ba{IN}10 meet the requirements for its potential use in phage therapy.},
  keywords = {{B}acteriophage ; {ABRC} ; v{A}b{IN}10 ; {C}haracterization ; {A}cinetobacter baumannii ; {AMR} ; {SENEGAL} ; {DAKAR}},
  booktitle = {},
  journal = {{J}ournal of {G}lobal {A}ntimicrobial {R}esistance},
  volume = {41},
  numero = {},
  pages = {151--158},
  ISSN = {2213-7165},
  year = {2025},
  DOI = {10.1016/j.jgar.2024.12.024},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092726},
}