@article{fdi:010092677,
  title = {{R}apid confirmation of autochthonous origin in suspected cases of melioidosis from {F}rench overseas departments in the {C}aribbean and the {I}ndian {O}cean by {PCR}-high resolution melting ({HRM}) analysis},
  author = {{G}asqu{\'e}, {M}{\'e}gane and {G}uernier, {V}anina and {G}irault, {G}. and {T}erret, {J}. and {N}eulat-{R}ipoll, {F}. and {R}ochelle-{N}ewall, {E}mma and {L}aroucau, {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}urkholderia pseudomallei, a soil-borne bacterium that causes melioidosis, endemic in {S}outh and {S}outheast {A}sia and northern {A}ustralia, is now emerging in new regions. {S}ince the 1990s, cases have been reported in {F}rench overseas departments, including {M}artinique and {G}uadeloupe in the {C}aribbean, and {R}eunion {I}sland and {M}ayotte in the {I}ndian {O}cean, suggesting a local presence of the bacterium. {O}ur phylogenetic analysis of 111 {B}. pseudomallei genomes isolated worldwide, including three strains from {M}artinique, revealed distinct geography-specific clades for {A}frica, the {A}mericas, {A}sia and {A}ustralasia. {S}ingle nucleotide polymorphisms ({SNP}) that define clade branches in the phylogeny were identified; we selected those specific to three regions relevant to the {F}rench overseas departments: the {I}ndian {O}cean, the {A}mericas and a unique subset specific to {M}artinique. {T}hree {SNP} markers (one per region) were used to develop a {PCR}-high resolution melting tool to discriminate between local and imported strains in each region. {B}lind tests on {B}. pseudomallei strains from {F}rench patients, from overseas departments and mainland {F}rance, were used for validation. {O}ur method accurately predicted the geographic origin of the patient as recorded from the patient travel history and/or from the multilocus sequence typing data. {T}his rapid typing method, which allows timely identification of local cases and targeted public health interventions, is particularly valuable in the {F}rench overseas departments where melioidosis is emerging and regulatory constraints limit the handling of {B}. pseudomallei. {A}lthough initially tailored to specific regions, this tool can be adapted for use in other areas to support local epidemiological surveillance of melioidosis.},
  keywords = {{M}elioidosis ; {G}enotyping ; {S}ingle nucleotide polymorphism ({SNP}s) ; {F}rench overseas territories ; {C}aribbean ; {I}ndian {O}cean ; {M}ultiple locus sequence typing ; {MARTINIQUE} ; {GUADELOUPE} ; {OCEAN} {INDIEN} ; {REUNION} ; {MAYOTTE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {127},
  numero = {},
  pages = {105711 [7 ]},
  ISSN = {1567-1348},
  year = {2025},
  DOI = {10.1016/j.meegid.2024.105711},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092677},
}