Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables Fioroni Nelly auteur aut IRD Rodríguez M. D. P. D. auteur aut IRD Leconte Nicolas auteur aut IRD Mouquet Rivier Claire auteur aut IRD Guzman C. auteur aut IRD Boudard F. auteur aut IRD Dhuique-Mayer C. auteur aut IRD Collin Myriam auteur aut IRD Deglos A. auteur aut IRD Reboul E. auteur aut IRD Bravo-Núñez A. auteur aut IRD Laurent-Babot C. auteur aut IRD text journalArticle eng text/pdf reformatted digital access The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells. Stimulation of this model with lipopolysaccharide/interferon-gamma resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. Polar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and THP-1 cells to different extents and restored barrier integrity and permeability. The developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses. specialized Caco-2/HT29 biculture THP-1 macrophages Leaf extract In vitro digestion Intestinal permeability Cytokines AFRIQUE SUBSAHARIENNE 054 050 NFS Journal 38 100213 [15 p.] 2025 2352-3646 https://www.documentation.ird.fr/hor/fdi:010092650 10.1016/j.nfs.2025.100213 2352-3646 [F B010092650] https://www.documentation.ird.fr/hor/fdi:010092650 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-03/010092650.pdf IRD - Base Horizon / Pleins textes 2025-03-12 2025-11-04 fdi:010092650 fre