Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES NFS Journal 100213 [15 p.] Caco-2/HT29 biculture THP-1 macrophages Leaf extract In vitro digestion Intestinal permeability Cytokines AFRIQUE SUBSAHARIENNE 2025 fdi:010092650 ENG NFS Journal 2352-3646 ISI:001405501000001 10.1016/j.nfs.2025.100213 https://www.documentation.ird.fr/hor/fdi:010092650 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-03/010092650.pdf 38 Horizon (IRD) The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells. Stimulation of this model with lipopolysaccharide/interferon-gamma resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. Polar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and THP-1 cells to different extents and restored barrier integrity and permeability. The developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses. 054 ; 050 UR267 / UR050 / UR232