%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Fioroni, Nelly
%A Rodríguez, M. D. P. D.
%A Leconte, Nicolas
%A Mouquet Rivier, Claire
%A Guzman, C.
%A Boudard, F.
%A Dhuique-Mayer, C.
%A Collin, Myriam
%A Deglos, A.
%A Reboul, E.
%A Bravo-Núñez, A.
%A Laurent-Babot, C.
%T Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables
%D 2025
%L fdi:010092650
%G ENG
%J NFS Journal
%@ 2352-3646
%K Caco-2/HT29 biculture ; THP-1 macrophages ; Leaf extract ; In vitro digestion ; Intestinal permeability ; Cytokines
%K AFRIQUE SUBSAHARIENNE
%M ISI:001405501000001
%P 100213 [15 ]
%R 10.1016/j.nfs.2025.100213
%U https://www.documentation.ird.fr/hor/fdi:010092650
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2025-03/010092650.pdf
%V 38
%W Horizon (IRD)
%X The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells. Stimulation of this model with lipopolysaccharide/interferon-gamma resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. Polar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and THP-1 cells to different extents and restored barrier integrity and permeability. The developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses.
%$ 054 ; 050