Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables /Fioroni, Nelly Rodríguez, M. D. P. D. /Leconte, Nicolas /Mouquet Rivier, Claire Guzman, C. Boudard, F. Dhuique-Mayer, C. /Collin, Myriam Deglos, A. Reboul, E. Bravo-Núñez, A. Laurent-Babot, C. Caco-2/HT29 biculture THP-1 macrophages Leaf extract In vitro digestion Intestinal permeability Cytokines The intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. In sub-Saharan Africa, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. Therefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal Caco-2 (colorectal adenocarcinoma) and HT29-MTX (goblet cell-like) together with the macrophage-like THP-1 cells. Stimulation of this model with lipopolysaccharide/interferon-gamma resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. Polar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and THP-1 cells to different extents and restored barrier integrity and permeability. The developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses. 2025 text https://www.documentation.ird.fr/hor/fdi:010092650 fdi:010092650 Fioroni Nelly, Rodríguez M. D. P. D., Leconte Nicolas, Mouquet Rivier Claire, Guzman C., Boudard F., Dhuique-Mayer C., Collin Myriam, Deglos A., Reboul E., Bravo-Núñez A., Laurent-Babot C.. Development of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two African green leafy vegetables. 2025, 38, 100213 [15 p.] EN AFRIQUE SUBSAHARIENNE