@article{fdi:010092650,
  title = {{D}evelopment of an in vitro triculture model of intestine inflammation to assess the immunomodulatory properties of cassava and roselle, two {A}frican green leafy vegetables},
  author = {{F}ioroni, {N}elly and {R}odríguez, {M}. {D}. {P}. {D}. and {L}econte, {N}icolas and {M}ouquet {R}ivier, {C}laire and {G}uzman, {C}. and {B}oudard, {F}. and {D}huique-{M}ayer, {C}. and {C}ollin, {M}yriam and {D}eglos, {A}. and {R}eboul, {E}. and {B}ravo-{N}úñez, {A}. and {L}aurent-{B}abot, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he intestine integrity and function are crucial for nutrient absorption and to prevent the entry of harmful antigens. {I}n sub-{S}aharan {A}frica, frequent infections and enteric dysfunction can cause intestinal inflammation that is associated with stunting. {T}herefore, to study the anti-inflammatory effects of cassava and roselle leaves, commonly consumed as sauces, we developed an in vitro triculture model of intestine inflammation using intestinal {C}aco-2 (colorectal adenocarcinoma) and {HT}29-{MTX} (goblet cell-like) together with the macrophage-like {THP}-1 cells. {S}timulation of this model with lipopolysaccharide/interferon-gamma resulted in mucus over-production, higher pro-inflammatory cytokine release and loss of intestinal barrier integrity due to increased permeability. {P}olar/non-polar extracts and digested sauces from cassava and roselle leaves reduced cytokine production in both intestinal and {THP}-1 cells to different extents and restored barrier integrity and permeability. {T}he developed and validated triculture model of inflamed intestine thus demonstrated the anti-inflammatory properties of cassava and roselle leaves, despite moderate responses.},
  keywords = {{C}aco-2/{HT}29 biculture ; {THP}-1 macrophages ; {L}eaf extract ; {I}n vitro digestion ; {I}ntestinal permeability ; {C}ytokines ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{NFS} {J}ournal},
  volume = {38},
  numero = {},
  pages = {100213 [15 p.]},
  ISSN = {2352-3646},
  year = {2025},
  DOI = {10.1016/j.nfs.2025.100213},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092650},
}