@article{fdi:010092617,
  title = {e{DNA}-based seasonal monitoring reveals fish diversity patterns in mangrove habitats of {G}uinea-{B}issau, {W}est {A}frica},
  author = {{K}eleman, {P}. {J}. and {D}urand, {J}ean-{D}ominique and {S}imier, {M}onique and {C}amará, {A}. and {S}á, {R}. {M}. and {P}anfili, {J}acques},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he environmental {DNA} (e{DNA}) technique characterizes spatio-temporal fish diversity patterns along estuarine habitats, such as hard-to-reach mangroves, yet it has rarely been applied in {W}est {A}frica. {I}n this study conducted over two seasons (dry and rainy) in coastal {G}uinea {B}issau, fish e{DNA} was extracted from water samples collected in three habitats with distinct human uses. {T}hese habitats included site-specific mangroves, rice fields, and transition zones sampled in three sites: {E}lalab in {C}acheu, {M}alafo in {O}io, and {C}afine in {T}ombali regions. {A}s a reference, {DNA} barcoding of 181 fishes resulted in 243 {DNA} sequences for two mitochondrial markers (139 for {COI} and 104 for 12{S}), corresponding to 76 species (69 for {COI} and 56 for 12{S}), after morpho-genetic confirmation. {T}he metabarcoding analysis detected 49 environmental {DNA}-{O}perational {T}axonomic {U}nits (e{OTU}s), attributed to 15 fish orders, 29 families, 38 genera, and 32 species. {F}ish abundance varied between sites and seasons but not between habitats. {T}he southern {C}afine site had a significantly lower abundance during the dry season, whereas the northern {E}lalab site hosted relatively fewer species during the rainy season. {T}he transition from rice fields to mangrove habitat showed a positive trend in {S}hannon diversity with no fish being present in the rice fields during the dry season except for {M}alafo. {R}emarkably, {C}optodon cf. guineensis, {S}arotherodon melanotheron and {P}seudotolithus elongatus dominated the fish assemblages. {T}his pioneering e{DNA} study efficiently surveyed seasonal fish diversity within {B}issau-{G}uinean mangroves, demonstrating its potential as a novel fish monitoring tool toward effective conservation in {W}est {A}frica. {T}he coupling of these results with the human uses of mangroves should be a target in future biomonitoring initiatives in the country and the broader region.},
  keywords = {{M}angrove {H}abitat {M}onitoring ; {M}etabarcoding ; {E}stuarine {B}iodiversity ; {S}easonal {F}ish {A}ssemblages ; {B}arcoding ; {GUINEE} {BISSAU}},
  booktitle = {},
  journal = {{R}egional {S}tudies in {M}arine {S}cience},
  volume = {82},
  numero = {},
  pages = {104013 [12 p.]},
  ISSN = {2352-4855},
  year = {2025},
  DOI = {10.1016/j.rsma.2025.104013},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092617},
}