@article{fdi:010092300,
  title = {{T}he genomic and transcriptomic basis of the potential of {L}actobacillus plantarum {A}6 to improve the nutritional quality of a cereal based fermented food},
  author = {{T}urpin, {W}. and {W}eiman, {M}. and {G}uyot, {J}ean-{P}ierre and {L}ajus, {A}. and {C}ruveiller, {S}. and {H}umblot, {C}hrist{\`e}le},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he objective of this work was to investigate the nutritional potential of {L}actobacillus plantarum {A}6 in a food matrix using next generation sequencing. {T}o this end, we characterized the genome of the {A}6 strain for a complete overview of its potential. {W}e then compared its transcriptome when grown in a food matrix made from pearl millet to and its transcriptome when cultivated in a laboratory medium. {G}enomic comparison of the strain {L}. plantarum {A}6 with the strains {WCFS}1, {ST}-{III}, {JDM}1 and {ATCC}14917 led to the identification of five regions of genomic plasticity. {M}ore specifically, 362 coding sequences, mostly annotated as coding for proteins of unknown functions, were specific to {L}. plantarum {A}6. {A} total of 1201 genes were significantly differentially expressed in laboratory medium and food matrix. {A}mong them, 821 genes were up-regulated in the food matrix compared to the laboratory medium, representing 23% of whole genomic objects. {I}n the laboratory medium, the expression of 380 genes, representing 11% of the all genomic objects was at least double than in the food matrix. {G}enes encoding important functions for the nutritional quality of the food were identified. {C}onsidering its efficiency as an amylolytic strain, we investigated all genes involved in carbohydrate metabolism, paying particular attention to starch metabolism. {A}n extracellular alpha amylase, a neopullulanase and maltodextrin transporters were identified, all of which were highly expressed in the food matrix. {I}n addition, genes involved in alpha-galactoside metabolism were identified but only two of them were induced in food matrix than in laboratory medium. {T}his may be because alpha galactosides were already eliminated during soaking. {D}ifferent biosynthetic pathways involved in the synthesis of vitamin {B} (folate, riboflavin, and cobalamin) were identified. {T}hey allowed the identification of a potential of vitamin synthesis, which should be confirmed through biochemical analysis in further work. {S}urprisingly, some genes involved in metabolism and bioaccessibility of iron were identified. {T}hey were related directly to the use of transport of iron, or indirectly to metabolism of polyphenols, responsible of iron chelation in the food. {T}he combination of genomics and transcriptomics not only revealed previously undocumented nutritional properties of {L}. plantarum {A}6, but also documented the behaviour of this bacterium in food.},
  keywords = {},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {F}ood {M}icrobiology},
  volume = {266},
  numero = {},
  pages = {346--354},
  ISSN = {0168-1605},
  year = {2018},
  DOI = {10.1016/j.ijfoodmicro.2017.10.011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092300},
}