@article{fdi:010092147,
  title = {{L}ineage-dependent partitioning of activities in chemoclines defines {W}oesearchaeota ecotypes in an extreme aquatic ecosystem},
  author = {{C}loarec, {L}. {A}. and {B}acchetta, {T}. and {B}ruto, {M}. and {L}eboulanger, {C}hristophe and {G}rossi, {V}. and {B}rochier-{A}rmanet, {C}. and {F}landrois, {J}. {P}. and {Z}urmely, {A}. and {B}ernard, {C}. and {T}roussellier, {M}. and {A}gogu{\'e}, {H}. and {A}der, {M}. and {O}ger-{D}esfeux, {C}. and {O}ger, {P}. {M}. and {V}igneron, {A}. and {H}ugoni, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {DPANN} archaea, including {W}oesearchaeota, encompass a large fraction of the archaeal diversity, yet their genomic diversity, lifestyle, and role in natural microbiomes remain elusive. {W}ith an archaeal assemblage naturally enriched in {W}oesearchaeota and steep vertical geochemical gradients, {L}ake {D}ziani {D}zaha ({M}ayotte) provides an ideal model to decipher their in-situ activity and ecology. {R}esults {U}sing genome-resolved metagenomics and phylogenomics, we identified highly diversified {W}oesearchaeota populations and defined novel halophilic clades. {D}epth distribution of these populations in the water column showed an unusual double peak of abundance, located at two distinct chemoclines that are hotspots of microbial diversity in the water column. {G}enome-centric metatranscriptomics confirmed this vertical distribution and revealed a fermentative activity, with acetate and lactate as end products, and active cell-to-cell processes, supporting strong interactions with other community members at chemoclines. {O}ur results also revealed distinct {W}oesearchaeota ecotypes, with different transcriptional patterns, contrasted lifestyles, and ecological strategies, depending on environmental/host conditions. {C}onclusions {T}his work provides novel insights into {W}oesearchaeota in situ activity and metabolism, revealing invariant, bimodal, and adaptative lifestyles among halophilic {W}oesearchaeota. {T}his challenges our precepts of an invariable host-dependent metabolism for all the members of this taxa and revises our understanding of their contributions to ecosystem functioning and microbiome assemblage.},
  keywords = {{A}rchaeal genomic diversity ; {W}oesearchaeales ; {E}cotypes ; {M}etagenomics ; {M}etatranscriptomics ; {E}xtreme lake ; {MAYOTTE}},
  booktitle = {},
  journal = {{M}icrobiome},
  volume = {12},
  numero = {1},
  pages = {249 [16 ]},
  ISSN = {2049-2618},
  year = {2024},
  DOI = {10.1186/s40168-024-01956-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092147},
}