@article{fdi:010092138,
  title = {{A}nalytical and clinical evaluation of a duplex {RT}-q{PCR} assay for the detection and identification of o'nyong-nyong and chikungunya virus},
  author = {{W}esselmann, {K}. {M}. and {L}uciani, {L}. and {T}hirion, {L}aurence and de {L}amballerie, {X}. and {C}harrel, {R}. and {P}ezzi, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he mosquito-borne alphavirus o'nyong-nyong virus ({ONNV}) has proven its potential to cause major human outbreaks. {O}n the {A}frican continent, {ONNV} causes unspecific febrile illness and co-circulates with the close relative chikungunya virus ({CHIKV}). {T}he true scale of {ONNV} burden is poorly understood in {A}frica, because of the scarce availability of molecular in-house and commercial assays, strong cross-reactivity between {ONNV} and {CHIKV} in serological assays and a lack of surveillance. {W}e designed a new {RT}-q{PCR} assay targeting the {E}1 gene for the detection of {ONNV} that can be used in monoplex or in duplex format, combined with a previously published {CHIKV} monoplex assay targeting the nsp1. {T}he lower limit of detection with 95% positivity rate was determined to be <10 {RNA} copies/<mu>{L} in monoplex and duplex format for both {ONNV} and {CHIKV}. {B}oth monoplex assays and the duplex assay proved to be linear within the tested range of approximately 108 to 102 {RNA} copies/mu l, and showed 100% specificity against a wide panel of arbovirus supernatants as well as several other pathogens in clinical samples. {T}esting of {CHIKV} positive serum and {ONNV}-spiked plasma samples confirmed the suitability of the assays in a clinical setting. {T}he new assays provide a robust tool for molecular {ONNV} as well as {ONNV}/{CHIKV} simultaneous detection and may contribute to clarify the true burden of the two viruses, to improve arbovirus surveillance and to strengthen epidemics preparedness in {A}frica.},
  keywords = {{A}lphavirus ; togaviridae ; arbovirus ; {C}hikungunya virus ; o'nyong-nyong virus ; diagnostics ; {RT}-q{PCR}},
  booktitle = {},
  journal = {{E}merging {M}icrobes and {I}nfections},
  volume = {13},
  numero = {1},
  pages = {2429650 [10 p.]},
  year = {2024},
  DOI = {10.1080/22221751.2024.2429650},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092138},
}