@article{fdi:010092077,
  title = {{W}arming and polymetallic stress induce proteomic and physiological shifts in the neurotoxic {A}lexandrium pacificum as possible response to global changes},
  author = {{J}ean, {N}. and {J}ames, {A}. and {B}alliau, {T}. and {M}artino, {C}. and {G}hersy, {J}. and {S}avar, {V}. and {L}aabir, {M}ohamed and {C}aruana, {A}. {M}. {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}armful {A}lgal {B}looms involving the dinoflagellate {A}lexandrium pacificum continue to increase in ecosystems suffering the climate warming and anthropogenic pressure. {C}hanges in the total proteome and physiological traits of the {M}editerranean {A}. pacificum {SG} {C}10-3 strain were measured in response to increasing temperature (24 degrees {C}, 27 degrees {C}, 30 degrees {C}) and trace metal contamination ({C}u2+, {P}b2+, {Z}n2+, {C}d2+). {W}arming reduced the cell densities and maximal growth rate (mu max), but the strain persisted at 30 degrees {C} with more large cells. {T}he polymetallic stress increased cell sizes, reduced cell growth at 24 degrees {C}-27 degrees {C} and it increased this at 30 degrees {C}. {T}oxin profiles showed a predominance of {GTX}4 (32-38 %), then {C}2 (11-34 %) or {GTX}6 (18-24 %) among the total {P}aralytic {S}hellfish {T}oxins, however these were modified under warming, showing increased contents in {GTX}1 (among the most toxic), {GTX}5, {C}1 and {N}eo{STX}, while dc-{N}eo{STX} and {STX} (among the most toxic) only appeared at 30 degrees {C}. {U}nder polymetallic contamination, warming also increased contents in {GTX}5 and {N}eo{STX}. {I}n contrast, polymetallic stress, or warming had harmful effects on {C}2 contents. {P}roteins were more quantitatively produced by {A}. pacificum {SG} {C}10-3 under warming in accordance with the high levels of up-regulated proteins found in the total proteome in this condition. {P}olymetallic stress, only or combined with warming, led to low proteomic modifications (1 % or 4 %), whereas warming induced strong 52 % modified proteomic response, mainly based on up-regulated proteins involved in photosynthesis (light harvesting complex protein), carbohydrate metabolism (arylsulfatase) and translation (ribosomal proteins), and with the lesser down-regulated proteins principally associated with the lipid metabolism (type {I} polyketide synthase). {O}ur results show that warming triggers a strong up-regulated {A}. pacificum {SG} {C}10-3 proteomic response, which, coupled to modified cell sizes and toxin profiles, could help it to withstand stress conditions. {T}his could presage the success of {A}. pacificum in anthropized ecosystems submitted to global warming in which this dinoflagellate also might be more toxic.},
  keywords = {{A}lexandrium pacificum ; {H}armful algal bloom ; {P}aralytic shellfish toxin ; {P}roteomics ; {G}lobal warming ; {T}race metals ; {MEDITERRANEE} ; {ITALIE} ; {SARDAIGNE}},
  booktitle = {},
  journal = {{M}arine {P}ollution {B}ulletin},
  volume = {209},
  numero = {{B}},
  pages = {117221 [19 p.]},
  ISSN = {0025-326{X}},
  year = {2024},
  DOI = {10.1016/j.marpolbul.2024.117221},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092077},
}