@article{fdi:010092056,
  title = {{C}haracterization of two {F}riunavirus phages and their inhibitory effects on biofilms of extremely drug resistant {A}cinetobacter baumannii in {D}akar, {S}enegal},
  author = {{N}diaye, {I}. and {D}ebarbieux, {L}. and {S}ow, {O}. and {B}a, {B}. {S}. and {D}iagne, {M}. {M}. and {C}iss{\'e}, {A}. and {F}all, {C}. and {D}ieye, {Y}. and {D}ia, {N}. and {C}onstantin de {M}agny, {G}uillaume and {S}eck, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {A}cinetobacter baumannii is a gram-negative, opportunistic pathogen, that is responsible for a wide variety of infections and is a significant cause of hospital-acquired infections. {A}. baumannii is listed by the {W}orld {H}ealth {O}rganization ({WHO}) as a critical priority pathogen because of its high level of antibiotic resistance and the urgent need for alternative treatment solutions. {T}o address this challenge, bacteriophages have been used to combat bacterial infections for more than a century, and phage research has regained interest in recent years due to antimicrobial resistance ({AMR}). {H}owever, although the vast majority of deaths from the {AMR} crisis will occur in developing countries in {A}frica and {A}sia, few phages' studies have been conducted in these regions. {I}n this study, we present a comprehensive characterization of the bacteriophages v{A}b{B}al23 and v{A}b{A}bd25, actives against extremely drug-resistant ({XDR}) {A}. baumannii. {M}ethods {P}hages were isolated from environmental wastewaters in {D}akar, {S}enegal. {T}he host-range, thermal and p{H} stabilities, infection kinetics, one step growth assay, antibiofilm activity assay, sequencing, and genomic analysis, were performed to characterize the isolated phages. {R}esults {C}omparative genomic and phylogenetic analyses revealed that v{A}b{B}al23 and v{A}b{A}bd25 belong to the {C}audoviricetes class, {A}utographiviridae family and {F}riunavirus genus. {B}oth phages demonstrated activity against strains with capsular type {KL}230. {T}hey were stable over a wide p{H} range (p{H} 3 to 9) and at temperatures ranging from 25 degrees {C} to 40 degrees {C}. {A}dditionally, the phages exhibited notable activity against both planktonic and biofilm cells of targeted extremely drug resistant {A}. baumannii. {T}he results presented here indicate the lytic nature of v{A}b{B}al23 and v{A}b{A}bd25. {T}his is further supported by the absence of genes encoding toxins, resistance genes and bacterial virulence factors, highlighting their potential for future phage applications. {C}onclusion {P}hages v{A}b{B}al23 and v{A}b{A}bd25 are promising biological agents that can infect {A}. baumannii, making them suitable candidates for use in phage therapies.},
  keywords = {{B}acteriophage ; v{A}b{B}al23 ; v{A}b{A}bd25 ; {C}haracterization ; {A}cinetobacter baumannii ; {A}ntimicrobial resistance ; {SENEGAL} ; {DAKAR}},
  booktitle = {},
  journal = {{BMC} {M}icrobiology},
  volume = {24},
  numero = {1},
  pages = {449 [11 ]},
  ISSN = {1471-2180},
  year = {2024},
  DOI = {10.1186/s12866-024-03608-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010092056},
}