@article{fdi:010091993,
  title = {{W}est {N}ile virus can be transmitted within mosquito populations through infectious mosquito excreta},
  author = {{H}amel, {R}odolphe and {N}arpon, {Q}. and {S}errato-{P}omar, {I}. and {G}auliard, {C}. and {B}erthomieu, {A}. and {W}ichit, {S}. and {M}iss{\'e}, {D}oroth{\'e}e and {S}ofonea, {M}. {T}. and {P}ompon, {J}ulien},
  editor = {},
  language = {{ENG}},
  abstract = {{U}nderstanding the transmission routes of arboviruses is key to determining their epidemiology. {H}ere, we tested whether {W}est {N}ile viruses ({WNV}s) are transmitted through mosquito excreta. {F}irst, we observed a high concentration of infectious units per excreta, although viruses were short lived. {S}econd, we showed that virion excretion starts early after oral infection and remains constant for a long period, regardless of mosquito infection level. {T}hese results highlight the infectiousness of excreta from infected mosquitoes. {T}hird, we found that both larvae and pupae were susceptible to infection, while pupae were highly permissive. {F}ourth, we established the proof-of-concept that immature mosquitoes can be infected by infectious excreta, demonstrating an excreta-mediated mode of transmission. {F}inally, by mathematically modeling excreta-mediated transmission in the field, we demonstrated that {WNV} can be transmitted within mosquito populations. {O}ur study uncovers a route of transmission for mosquito-borne arboviruses, unveiling mechanisms of viral maintenance in mosquito reservoirs.},
  keywords = {infected mosquitoes ; {M}osquito aquatic stages ; excreta ; excreta-mediated},
  booktitle = {},
  journal = {i{S}cience},
  volume = {27},
  numero = {11},
  pages = {111099 [16 p.]},
  year = {2024},
  DOI = {10.1016/j.isci.2024.111099},
  URL = {https://www.documentation.ird.fr/hor/fdi:010091993},
}