@article{fdi:010091626,
  title = {{C}omparative transcriptomics reveals a highly polymorphic {X}anthomonas {H}rp{G} virulence regulon},
  author = {{M}onnens, {T}.{Q}. and {R}oux, {B}. and {C}unnac, {S}{\'e}bastien and {C}harbit, {E}. and {C}arr{\`e}re, {S}. and {L}auber, {E}. and {J}ardinaud, {M}.{F}. and {D}arrasse, {A}. and {A}rlat, {M}. and {S}zurek, {B}oris and {P}ruvost, {O}. and {J}acques, {M}.{A}. and {G}agnevin, {L}. and {K}oebnik, {R}alf and {N}o{\¨e}l, {L}.{D}. and {B}oulanger, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground : {B}acteria of the genus {X}anthomonas cause economically significant diseases in various crops. {T}heir virulence is dependent on the translocation of type {III} effectors ({T}3{E}s) into plant cells by the type {III} secretion system ({T}3{SS}), a process regulated by the master response regulator {H}rp{G}. {A}lthough {H}rp{G} has been studied for over two decades, its regulon across diverse {X}anthomonas species, particularly beyond type {III} secretion, remains understudied. {R}esults : {I}n this study, we conducted transcriptome sequencing to explore the {H}rp{G} regulons of 17 {X}anthomonas strains, encompassing six species and nine pathovars, each exhibiting distinct host and tissue specificities. {W}e employed constitutive expression of plasmid-borne hrp{G}*, which encodes a constitutively active form of {H}rp{G}, to induce the regulon. {O}ur findings reveal substantial inter- and intra-specific diversity in the {H}rp{G}* regulons across the strains. {B}esides 21 genes directly involved in the biosynthesis of the {T}3{SS}, the core {H}rp{G}* regulon is limited to only five additional genes encoding the transcriptional activator {H}rp{X}, the two {T}3{E} proteins {X}op{R} and {X}op{L}, a major facility superfamily ({MFS}) transporter, and the phosphatase {P}ho{C}. {I}nterestingly, genes involved in chemotaxis and genes encoding enzymes with carbohydrate-active and proteolytic activities are variably regulated by {H}rp{G}*. {C}onclusions : {T}he diversity in the {H}rp{G}* regulon suggests that {H}rp{G}-dependent virulence in {X}anthomonas might be achieved through several distinct strain-specific strategies, potentially reflecting adaptation to diverse ecological niches. {T}hese findings enhance our understanding of the complex role of {H}rp{G} in regulating various virulence and adaptive pathways, extending beyond {T}3{E}s and the {T}3{SS}.},
  keywords = {},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {25},
  numero = {1},
  pages = {777 [17 ]},
  ISSN = {1471-2164},
  year = {2024},
  DOI = {10.1186/s12864-024-10684-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010091626},
}