@article{fdi:010091340, title = {{N}itrate and ammonium, the yin and yang of nitrogen uptake : a time-course transcriptomic study in rice}, author = {{P}elissier, {P}. {M}. and {P}arizot, {B}. and {J}ia, {L}. and {D}e {K}nijf, {A}. and {G}oossens, {V}. and {G}antet, {P}ascal and {C}hampion, {A}ntony and {A}udenaert, {D}. and {X}uan, {W}. and {B}eeckman, {T}. and {M}otte, {H}.}, editor = {}, language = {{ENG}}, abstract = {{N}itrogen is an essential nutrient for plants and a major determinant of plant growth and crop yield. {P}lants acquire nitrogen mainly in the form of nitrate and ammonium. {B}oth nitrogen sources affect plant responses and signaling pathways in a different way, but these signaling pathways interact, complicating the study of nitrogen responses. {E}xtensive transcriptome analyses and the construction of gene regulatory networks, mainly in response to nitrate, have significantly advanced our understanding of nitrogen signaling and responses in model plants and crops. {I}n this study, we aimed to generate a more comprehensive gene regulatory network for the major crop, rice, by incorporating the interactions between ammonium and nitrate. {T}o achieve this, we assessed transcriptome changes in rice roots and shoots over an extensive time course under single or combined applications of the two nitrogen sources. {T}his dataset enabled us to construct a holistic co-expression network and identify potential key regulators of nitrogen responses. {N}ext to known transcription factors, we identified multiple new candidates, including the transcription factors {O}s{RLI} and {O}s{EIL}1, which we demonstrated to induce the primary nitrate-responsive genes {O}s{NRT}1.1b and {O}s{NIR}1. {O}ur network thus serves as a valuable resource to obtain novel insights in nitrogen signaling.}, keywords = {transcriptome ; rice ; co-expression network ; nitrogen ; {O}s{RLI}1 ; {O}s{EIL}1}, booktitle = {}, journal = {{F}rontiers in {P}lant {S}cience}, volume = {15}, numero = {}, pages = {1343073 [16 p.]}, ISSN = {1664-462{X}}, year = {2024}, DOI = {10.3389/fpls.2024.1343073}, URL = {https://www.documentation.ird.fr/hor/fdi:010091340}, }