@article{fdi:010091269,
  title = {{ESBL}-producing {E}scherichia coli prevalence and sharing across seabirds of central {C}hile},
  author = {{S}uarez-{Y}ana, {T}. and {S}algado-{C}axito, {M}. and {H}ayer, {J}uliette and {R}ojas-{S}ereno, {Z}. {E}. and {P}ino-{H}urtado, {M}. {S}. and {C}ampaña-{B}urguet, {A}. and {C}aparrós, {C}. and {T}orres, {C}. and {B}enavides, {J}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ntimicrobial resistance ({AMR}) is a major global public threat, now largely reported in natural environments. {S}eabirds are carriers of extended-spectrum beta-lactamase-producing {E}scherichia coli ({ESBL}-{E}. coli), but different foraging and breeding behaviour could impact {ESBL}-{E}. coli circulation. {W}e compared the prevalence and genetic determinants of {ESBL}-{E}. coli from resident {K}elp gulls ({L}arus dominicanus, {L}d), migratory {F}ranklin's gulls ({L}arus pipixcan, {L}p), and endemic {P}eruvian pelicans ({P}elecanus thagus, {P}t) from the {H}umboldt {C}urrent {E}cosystem ({HCE}) of central {C}hile. {F}rom 2020 to 2022, we collected 699 fresh faecal samples ({L}d = 449, {L}p = 116, {P}t = 134), and isolated 271 {ESBL}-{E}. coli (39 %). {W}hole-genome-sequencing ({WGS}) was performed on 85 {E}. coli selected isolates to identify their {S}equence {T}ype ({ST}), {AMR} genes, virulence genes, mobile genetic elements ({MGE}), and to assess potential interspecies transmission. {ESBL}-genes were detected in the remaining {ESBL}-{E}. coli isolates by {PCR}. {ESBL}-{E}. coli prevalence in {L}d (46 % [{CI}: 42-51 %]) and {P}t (34 % [{CI}: 27-43 %]) was higher than in {L}p (15 % [{CI}: 9-22 %]). {WGS} revealed 41 {ESBL}-{E}. coli {ST}s including pandemic clones {ST}10, {ST}58, {ST}131 and {ST}410. {T}he bla{CTX}-{M}-1 and bla{CTX}-{M}-15 genes were the most prevalent among {ESBL} genes, and were mostly associated with {MGE} {I}nc{I}1-{I}({A}lpha) and {ISE}c9. {W}e also report the p{A}mp{C} bla{CMY}-2 gene associated to {MGE} {I}nc1-{I}({A}lpha) and {IS}640 in two {E}. coli from a {L}d and a {L}p. {E}ight {ESBL}-{E}. coli of the same {ST} were shared by at least two seabird species, including {ST}10 ({L}d and {P}t); {ST}88, {ST}410 and {ST}617 ({P}t and {L}p); {ST}38, {ST}58, {ST}131, and {SST}1722 (three species). {S}ingle nucleotide polymorphism ({SNP}) phylogenetic analyses of {ST}38, {ST}617 and {ST}1722 showed a low difference of {SNP}s between {ST}s found in different seabird species, suggesting {ESBL}-{E}. coli clonal exchanges. {O}ur results highlight {ESBL}-{E}. coli dissemination across seabirds of the {HCE}, including species that unusually forage on human waste like pelicans.},
  keywords = {{L}atin {A}merica ; {A}ntimicrobial resistance ; {G}ulls ; {P}elicans ; {W}hole genome sequencing ; {CHILI}},
  booktitle = {},
  journal = {{S}cience of the {T}otal {E}nvironment},
  volume = {951},
  numero = {},
  pages = {175475 [13 p.]},
  ISSN = {0048-9697},
  year = {2024},
  DOI = {10.1016/j.scitotenv.2024.175475},
  URL = {https://www.documentation.ird.fr/hor/fdi:010091269},
}