@article{fdi:010091247,
  title = {{P}revalence of dermal trypanosomes in suspected and confirmed cases of gambiense human {A}frican trypanosomiasis in {G}uinea},
  author = {{S}oumah, {A}. {M}. and {C}amara, {M}. and {K}abor{\'e}, {J}. {W}. and {S}adissou, {I}brahim and {I}lboudo, {H}. and {T}ravaill{\'e}, {C}. and {C}amara, {O}. and {T}ichit, {M}. and {K}abor{\'e}, {J}. and {B}oiro, {S}. and {C}rouzols, {A}. and {N}goune, {J}. {M}. {T}. and {H}ardy, {D}. and {C}amara, {A}. and {J}amonneau, {V}incent and {M}ac{L}eod, {A}. and {B}art, {J}ean-{M}athieu and {C}amara, {M}. and {B}ucheton, {B}runo and {R}otureau, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he skin is an anatomical reservoir for {A}frican trypanosomes, yet the prevalence of extravascular parasite carriage in the population at risk of gambiense {H}uman {A}frican {T}rypanosomiasis (g{HAT}) remains unclear. {H}ere, we conducted a prospective observational cohort study in the {HAT} foci of {F}orecariah and {B}offa, {R}epublic of {G}uinea. {O}f the 18,916 subjects serologically screened for g{HAT}, 96 were enrolled into our study. {A}t enrolment and follow-up visits, participants underwent a dermatological examination and had blood samples and superficial skin snip biopsies taken for examination by molecular and immuno-histological methods. {I}n seropositive individuals, dermatological symptoms were significantly more frequent as compared to seronegative controls. {T}rypanosoma brucei {DNA} was detected in the blood of 67% of confirmed cases (22/33) and 9% of unconfirmed seropositive individuals (3/32). {H}owever, parasites were detected in the extravascular dermis of up to 71% of confirmed cases (25/35) and 41% of unconfirmed seropositive individuals (13/32) by {PCR} and/or immuno-histochemistry. {S}ix to twelve months after treatment, trypanosome detection in the skin dropped to 17% of confirmed cases (5/30), whereas up to 25% of unconfirmed, hence untreated, seropositive individuals (4/16) were still found positive. {D}ermal trypanosomes were observed in subjects from both transmission foci, however, the occurrence of pruritus and the {PCR} positivity rates were significantly higher in unconfirmed seropositive individuals in {F}orecariah. {T}he lower sensitivity of superficial skin snip biopsies appeared critical for detecting trypanosomes in the basal dermis. {T}hese results are discussed in the context of the planned elimination of g{HAT}.},
  keywords = {{GUINEE}},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {18},
  numero = {8},
  pages = {e0012436 [20 p.]},
  ISSN = {1935-2735},
  year = {2024},
  DOI = {10.1371/journal.pntd.0012436},
  URL = {https://www.documentation.ird.fr/hor/fdi:010091247},
}