@article{fdi:010091160,
  title = {{A}nalyzing cervical microbiome composition in {HIV}-infected women with different {HPV} infection profiles : a pilot study in {T}hailand},
  author = {{P}reechasuth, {K}. and {B}razier, {L}. and {K}hamduang, {W}. and {H}ongjaisee, {S}. and {W}angsaeng, {N}. and {N}go-{G}iang-{H}uong, {N}icole},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e conducted a pilot study to analyze the microbiome in cervical samples of women living with {HIV} with various profiles of {HPV} infections. {T}he participants had an average age of 41.5 years. {S}equence analysis of 16{S} r{RNA} {V}3 gene amplicons was performed using next-generation sequencing technology ({I}on {T}orrent {PGMTM}). {T}he bioinformatics pipeline was analyzed using the {F}ind, {R}apidly, {OTU}s with {G}alaxy {S}olution system ({FROGS}). {C}ommon genera were determined to identify {C}ommunity {S}tate {T}ypes ({CST}s). {T}he cervical microbiome profiles showed a dominance of lactobacilli in 56% (five out of nine) of samples. {A}ll three women with normal cervical cells and high-risk {HPV} infection were classified as {CST} {IV}, characterized by anaerobic bacteria associated with bacterial vaginitis, such as {G}ardnerella, {P}revotella, {A}topobium, and {S}neathia. {A}mong the two women with abnormal cervical cells and high-risk {HPV} infection, one was classified as {CST} {III}, and the other had an unclassified profile dominated by {L}. helveticus. {F}our women with normal cervical cells and no {HPV} infection exhibited various {CST}s. {O}ur study demonstrated the feasibility of the protocol in analyzing the cervical microbiome. {H}owever, further analysis with a larger number of longitudinal samples is necessary to determine the role of cervical microbiota in {HPV} persistence, clearance, or the development of precancerous lesions.},
  keywords = {vaginal microbiome ; human papillomavirus ; {NGS} ; community state types ; {THAILANDE}},
  booktitle = {},
  journal = {{M}icroorganisms},
  volume = {12},
  numero = {7},
  pages = {1298 [8 ]},
  year = {2024},
  DOI = {10.3390/microorganisms12071298},
  URL = {https://www.documentation.ird.fr/hor/fdi:010091160},
}