@article{fdi:010091145, title = {{U}pscaling irradiation protocols of {A}edes albopictus pupae within an {SIT} program in {R}eunion {I}sland}, author = {{M}arquereau, {L}. and {Y}amada, {H}. and {D}amiens, {D}. and {L}eclercq, {A}. and {D}erepas, {B}. and {B}rengues, {C}{\'e}cile and {D}ain, {B}. {W}. and {L}ejarre, {Q}. and {P}roudhon, {M}. and {B}ouyer, {J}. and {G}ouagna, {L}ouis-{C}l{\'e}ment}, editor = {}, language = {{ENG}}, abstract = {{T}he implementation of the sterile insect technique against {A}edes albopictus relies on many parameters, in particular on the success of the sterilization of males to be released into the target area in overflooding numbers to mate with wild females. {A}chieving consistent sterility levels requires efficient and standardized irradiation protocols. {H}ere, we assessed the effects of exposure environment, density of pupae, irradiation dose, quantity of water and location in the canister on the induced sterility of male pupae. {W}e found that the irradiation of 2000 pupae in 130 ml of water and with a dose of 40 {G}y was the best combination of factors to reliably sterilize male pupae with the specific irradiator used in our control program, allowing the sterilization of 14000 pupae per exposure cycle. {T}he location in the canister had no effect on induced sterility. {T}he results reported here allowed the standardization and optimization of irradiation protocols for a {S}terile {I}nsect {T}echnique program to control {A}e. albopictus on {R}eunion {I}sland, which required the production of more than 300,000 sterile males per week.}, keywords = {{S}terile insect technique ; {U}pscale ; {I}rradiation method ; {A}edes albopictus ; {REUNION}}, booktitle = {}, journal = {{S}cientific {R}eports - {N}ature}, volume = {14}, numero = {1}, pages = {12117 [9 p.]}, ISSN = {2045-2322}, year = {2024}, DOI = {10.1038/s41598-024-62642-7}, URL = {https://www.documentation.ird.fr/hor/fdi:010091145}, }