@article{fdi:010091090,
  title = {{U}ptake of dissolved inorganic nitrogen and {N}2 fixation by {C}rocosphaera watsonii under climate change scenarios},
  author = {{U}mbricht, {J}. and {F}ilella, {A}. and {K}lett, {A}. and {V}ogts, {A}. and {B}enavides, {M}ar and {V}oss, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he response of {N}-2 fixation to projected future conditions in the ocean cannot be reliably predicted to date. {W}e conducted a minicosm experiment with pre-acclimated cultures of the globally significant diazotroph {C}rocosphaera watsonii strain {WH}8501 ("{C}rocosphaera"). {PH} and temperature were altered simultaneously to match the {RCP} scenarios 4.5 and 6 and investigate a more realistic future scenario compared to studies that focus on changes of a single stressor only. {T}he cell abundance and nitrogen metabolism of {C}rocosphaera was monitored over 5 days. {O}ur results imply that {C}rocosphaera is able to simultaneously perform {N}-2 fixation and assimilate dissolved inorganic nitrogen ({DIN}, i.e., nitrate and ammonium) under all the conditions tested and implies a competition with non-diazotrophic phytoplankton for {DIN}, which should be further investigated. {U}sing {N}ano{SIMS} analysis of single cells, our results point towards a preference for {DIN} assimilation over {N}-2 fixation under more acidic and warmer conditions. {O}verall, our results show that while the combined alteration of p{H} and temperature had a negative effect on the diazotroph's growth and {N}-2 fixation, {C}rocosphaera is likely to cope well with conditions in the future ocean. {T}he high intra-population variability in nitrogen assimilation pathways may give this species the flexibility to quickly react to environmental changes.},
  keywords = {nitrogen fixation ; ocean warming ; ocean acidification ; nitrogen assimilation ; {C}rocosphaera ; minicosm experiment ; {MEDITERRANEE}},
  booktitle = {},
  journal = {{F}rontiers in {M}arine {S}cience},
  volume = {11},
  numero = {},
  pages = {1388214 [13 p.]},
  year = {2024},
  DOI = {10.3389/fmars.2024.1388214},
  URL = {https://www.documentation.ird.fr/hor/fdi:010091090},
}