Hounmenou C. G. auteur aut IRD Le Marcis Frédéric auteur aut IRD Kaba D. auteur aut IRD Diaby M. auteur aut IRD Soumah A. K. auteur aut IRD Diallo H. auteur aut IRD Thaurignac G. auteur aut IRD Camara S. C. auteur aut IRD Ayouba Ahidjo auteur aut IRD Peeters Martine auteur aut IRD Keita A. K. auteur aut IRD Delaporte E. auteur aut IRD Toure A. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Objectives: This study aimed to investigate the prevalence of orthoebolavirus antibodies in Madina Oula, a non-epidemic rural area in Guinea, in 2022. Methods: A cross-sectional study was conducted from March 14 to April 3, 2022 involving recording household and socio-demographic characteristics, lifestyle data, and collecting dried blood spots from 878 individuals in 235 households. Dried blood spots were tested using multiplex serology to detect antibodies to different orthoebolaviruses : Ebola virus, Bundibugyo virus, Sudan virus, Reston virus, and Bombali virus. Seroprevalence was estimated with a 95% confidence interval and a Z-test was performed to compare the seropositivity between children aged under 15 years and those over 15 years . Household and participant characteristics were analyzed using descriptive statistic, and socio-historical conditions were discussed. Results: The serological analysis conducted in 2022 on 878 participants revealed varying reactivity to orthoebolavirus antigens, notably, with glycoprotein antigens, particularly, glycoprotein Sudan virus (16%). A total of 21 samples exhibited reactivity with at least two antigens, with a median age of 27 years (interquartile range 10.0 0-35.0 0), ranging from 2 to 80 years. There is no significant difference between seropositivity in children aged under 15 (2.86%) years and those over 15 (2.14%) years. The antibody presence varied per village, with the highest prevalence observed in Ouassou and Dar-es-Salam. Conclusions: Serological data in a region unaffected by recent Ebola outbreaks indicate possible orthoebolavirus endemicity, emphasizing the need for preparedness against known or novel orthoebolaviruses with potential cross-reactivity. specialized Ebola virus disease Seroprevalence Guinea rural population Outbreak Surveillance strategies GUINEE 050 052 146 107129 [8 ] 2024 1201-9712 https://www.documentation.ird.fr/hor/fdi:010091079 10.1016/j.ijid.2024.107129 1201-9712 [F B010091079] https://www.documentation.ird.fr/hor/fdi:010091079 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-09/010091079.pdf IRD - Base Horizon / Pleins textes 2024-08-28 2025-11-06 fdi:010091079 fre