Gimonneau G. auteur aut IRD Buatois B. auteur aut IRD Lapeyre B. auteur aut IRD Salou E. W. auteur aut IRD Sanon N. auteur aut IRD Ranaivoarisoa A. auteur aut IRD Roux Olivier auteur aut IRD Dormont L. auteur aut IRD text journalArticle eng text/pdf born digital access Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of human and animal trypanosomes. This viviparous insect develops and produces a single larva at 10-day intervals deposited in specific sites. In some species aggregation of larvae has been shown and seems to be mediated by both physical factors and volatile semiochemicals of larval origin. In this context, this study aims to identify chemicals emitted during the pupariation process in Glossina palpalis gambiensis. Volatile Organic Compounds (VOCs) emitted by larvae were identified using static headspace solid-phase microextraction and gas-chromatography mass-spectrometry (GC-MS) analysis. Electrophysiology and behavioural assays were performed on gravid females to confirm VOCs behavioural activity and attractiveness. GC-MS results revealed ten chemicals emitted during the pupariation process of G. p. gambiensis larvae. Among these chemicals, gravid females were shown to detect nine of them during coupled gas chromatography - electroantennographic detection tests. Behavioural assays highlighted two compounds were as attractive as pupae and one compound and a blend of four compounds were more attractive than pupae. Although the larval origin of some of them needs to be confirmed as they may also likely produced by micro-organisms, these compounds induced significant behavioural responses in the laboratory. Further experiments have to explore the biological activity and competitiveness of these compounds in the field. This work opens interesting opportunities for behavioural manipulation and control of tsetse flies. specialized Tsetse fly Reproduction Chemical ecology Larviposition Aggregation 052 020 [Early access] [14 p.] 2024 0098-0331 https://www.documentation.ird.fr/hor/fdi:010091010 10.1007/s10886-024-01524-8 0098-0331 [F B010091010] https://www.documentation.ird.fr/hor/fdi:010091010 https://www.documentation.ird.fr/intranet/publi/2024-08/010091010.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2024-08-28 2025-11-06 fdi:010091010 fre