@inproceedings{fdi:010090894, title = {{E}ffect of {U}lva supplemented feeds, with and without probiotic ({D}ebaryomyces hansenii) supplementation, on the growth, immunity, oxidative stress response and gut morphology/microbiome of the dusky kob {A}rgyrosomus japonicus [r{\'e}sum{\'e}]}, author = {{K}utu, {V}. and {M}acey, {B}.{M}. and {D}arias, {M}aria {J}.}, editor = {}, language = {{ENG}}, abstract = {{G}lobally, several seaweed species are reported to have great potential as functional ingredients in aquafeed for a variety of fish species, including {S}olea senegalensis, {C}larias gariepinus, {S}almo salar and {O}reochromis niloticus. {U}lva has been cultivated by the {S}outh {A}frican abalone industry in {I}ntegrated {M}ulti-trophic {A}quaculture ({IMTA}) systems for use as feedstock or crop-based dietary ingredients in formulated feeds for aquacultured abalone ({H}aliotis midae) for almost two decades, and more recently for sea urchins ({T}ripneustes gratilla and {P}arechinus angulosus). {T}he current study aims to enhance the growth, immunity, oxidative stress, gut morphology and microbiome of {A}rgyrosomus japonicus (dusky kob) through dietary supplementation with dried {U}lva lacinulata. {D}ried {U}. lacinulata inclusion levels of 5, 10 and 15% (w/w) will be tested and compared with a non-supplemented (0% {U}lva) control feed, totalling to four (4) dietary treatments- of 3 replicate tanks each. {D}ata will be collected for (1) growth performance, and nutrient utilization: {W}eight gain, feed conversion ratio, specific growth rate, condition factor, hepatosomatic index, viscera-somatic index and survival rate; (2) {H}aematology: blood glucose, haematocrit and blood histology; (3) {I}mmune response by assessing variation in the expression of relevant immune genes such as {IL}-1 b{\^e}ta, {IL}-10, {TNF}-alpha and {I}g{M} from selected tissue samples (liver, intestine, head kidney and spleen). {T}his will be conducted using semi-quantitative {R}eal-{T}ime {P}olymerase {C}hain {R}eaction ({RT}-{PCR}); (4) {F}lesh proximate analysis; (5) {A}ntioxidant indicators of serum and liver; (6) {H}istology of the intestine; (7) {M}icrobiome associated with gut tissue. {R}esults from this study will help inform the aquaculture industry about how dietary {U}lva-supplementation can help improve the health and welfare of one of {S}outh {A}frica's promising aquacultured fish species, the dusky kob {A}. japonicus. {T}he presentation will provide an overview of the work that is planned and currently underway.}, keywords = {{AFRIQUE} {DU} {SUD} ; {ATLANTIQUE} ; {OCEAN} {INDIEN}}, numero = {}, pages = {59}, booktitle = {{A}fri{MAQUA} 2023 {C}onference : towards a more sustainable aquatic food system : interdisciplinary research on sustainable marine aquaculture in {A}frica : book of abstracts}, year = {2023}, URL = {https://www.documentation.ird.fr/hor/fdi:010090894}, }