@article{fdi:010090757, title = {{H}aitian coffee agroforestry systems harbor complex arabica variety mixtures and under-recognized genetic diversity}, author = {{M}illet, {C}. {P}. and {A}llinne, {C}. and {V}i, {T}. and {M}arraccini, {P}. and {V}erleysen, {L}. and {C}ouderc, {M}arie and {R}uttink, {T}. and {Z}hang, {D}. {P}. and {S}anchez, {W}. {S}. and {D}ubreuil {T}ranchant, {C}hristine and {J}eune, {W}. and {P}oncet, {V}al{\'e}rie}, editor = {}, language = {{ENG}}, abstract = {{T}hough facing significant challenges, coffee ({C}offea arabica) grown in {H}aitian agroforestry systems are important contributors to rural livelihoods and provide several ecosystem services. {H}owever, little is known about their genetic diversity and the variety mixtures used. {I}n light of this, there is a need to characterize {H}aitian coffee diversity to help inform revitalization of this sector. {W}e sampled 28 diverse farms in historically important coffee growing regions of northern and southern {H}aiti. {W}e performed {KASP}-genotyping of {SNP} markers and {H}i{P}lex multiplex amplicon sequencing for haplotype calling on our samples, as well as several {E}thiopian and commercial accessions from international collections. {T}his allowed us to assign {H}aitian samples to varietal groups. {O}ur analyses revealed considerable genetic diversity in {H}aitian farms, higher in fact than many farmers realized. {N}otably, genetic structure analyses revealed the presence of clusters related to {T}ypica, {B}ourbon, and {C}atimor groups, another group that was not represented in our reference accession panel, and several admixed individuals. {A}cross the study areas, we found both mixed-variety farms and monovarietal farms with the historical and traditional {T}ypica variety. {T}his study is, to our knowledge, the first to genetically characterize {H}aitian {C}. arabica variety mixtures, and report the limited cultivation of {C}. canephora ({R}obusta coffee) in the study area. {O}ur results show that some coffee farms are repositories of historical, widely-abandoned varieties while others are generators of new diversity through genetic mixing.}, keywords = {{HAITI}}, booktitle = {}, journal = {{PL}o{S} {O}ne}, volume = {19}, numero = {4}, pages = {e0299493 [24 p.]}, ISSN = {1932-6203}, year = {2024}, DOI = {10.1371/journal.pone.0299493}, URL = {https://www.documentation.ird.fr/hor/fdi:010090757}, }