@article{fdi:010090755,
  title = {{P}otential of {MALDI}-{TOF} {MS} biotyping to detect deltamethrin resistance in the dengue vector {A}edes aegypti},
  author = {{A}lmeras, {L}. and {C}osta, {M}. {M}. and {A}malvict, {R}. and {G}uilliet, {J}. and {D}usfour, {I}. and {D}avid, {J}. {P}. and {C}orbel, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}nsecticide resistance in mosquitoes is spreading worldwide and represents a growing threat to vector control. {I}nsecticide resistance is caused by different mechanisms including higher metabolic detoxication, target-site modification, reduced penetration and behavioral changes that are not easily detectable with simple diagnostic methods. {I}ndeed, most molecular resistance diagnostic tools are costly and labor intensive and then difficult to use for routine monitoring of insecticide resistance. {T}he present study aims to determine whether mosquito susceptibility status against the pyrethroid insecticides (mostly used for mosquito control) could be established by the protein signatures of legs and/or thoraxes submitted to {MALDI}-{TOF} {M}ass {S}pectrometry ({MS}). {T}he quality of {MS} spectra for both body parts was controlled to avoid any bias due to unconformity protein profiling. {T}he comparison of {MS} profiles from three inbreeds {A}e. aegypti lines from {F}rench {G}uiana ({IRF}, {IR}03, {IR}13), with distinct deltamethrin resistance genotype / phenotype and the susceptible reference laboratory line {BORA} ({F}rench {P}olynesia), showed different protein signatures. {O}n both body parts, the analysis of whole protein profiles revealed a singularity of {BORA} line compared to the three inbreeding lines from {F}rench {G}uiana origin, suggesting that the first criteria of differentiation is the geographical origin and/or the breeding history rather than the insecticide susceptibility profile. {H}owever, a deeper analysis of the protein profiles allowed to identify 10 and 11 discriminating peaks from leg and thorax spectra, respectively. {A}mong them, a specific peak around 4870 {D}a was detected in legs and thoraxes of pyrethroid resistant lines compared to the susceptible counterparts hence suggesting that {MS} profiling may be promising to rapidly distinguish resistant and susceptible phenotypes. {F}urther work is needed to confirm the nature of this peak as a deltamethrin resistant marker and to validate the routine use of {MS} profiling to track insecticide resistance in {A}e. aegypti field populations.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {19},
  numero = {5},
  pages = {e0303027 [18 ]},
  ISSN = {1932-6203},
  year = {2024},
  DOI = {10.1371/journal.pone.0303027},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090755},
}