@article{fdi:010090738,
  title = {{R}ole of endogenous soil microorganisms in controlling antimicrobial resistance after the exposure to treated wastewater},
  author = {{D}ella-{N}egra, {O}. and {B}astos, {M}. {C}. and {B}ru-{A}dan, {V}. and {S}anta-{C}atalina, {G}. and {A}it-{M}ouheb, {N}. and {C}hiron, {S}erge and {H}eran, {M}. and {W}{\'e}ry, {N}. and {P}atureau, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he reuse of treated wastewater ({TWW}) for irrigation appears to be a relevant solution to the challenges of growing water demand and scarcity. {H}owever, {TWW} contains not only micro-pollutants including pharmaceutical residues but also antibiotic resistant bacteria. {T}he reuse of {TWW} could contribute to the dissemination of antimicrobial resistance in the environment. {T}he purpose of this study was to assess if exogenous bacteria from irrigation waters ({TWW} or tap water-{TP}) affect endogenous soil microbial communities (from 2 soils with distinct irrigation history) and key antibiotic resistance gene sul1 and mobile genetic elements intl1 and {IS} 613 . {E}xperiments were conducted in microcosms, irrigated in one-shot, and monitored for three months. {R}esults showed that {TP} or {TWW} exposure induced a dynamic response of soil microbial communities but with no significant increase of resistance and mobile gene abundances. {H}owever, no significant differences were observed between the two water types in the current experimental design. {D}espite this, the 16{S} r{DNA} analysis of the two soils irrigated for two years either with tap water or {TWW} resulted in soil microbial community differentiation and the identification of biomarkers from {X}anthomonadaceae and {P}lanctomycetes families for soils irrigated with {TWW}. {L}ow-diversity soils were more sensitive to the addition of {TWW}. {I}ndeed, {TWW} exposure stimulated the growth of bacterial genera known to be pathogenic, correlating with a sharp increase in the copy number of selected resistance genes (up to 3 logs). {T}hese low -diversity soils could thus enable the establishment of exogenous bacteria from {TWW} which was not observed with native soils. {I}n particular, the emergence of {P}lanctomyces, previously suggested as a biomarker of soil irrigated by {TWW}, was here demonstrated. {F}inally, this study showed that water input frequency, initial soil microbial diversity and soil history drive changes within soil endogenous communities and the antibiotic resistance gene pool.},
  keywords = {{A}ntimicrobial resistance ; {W}ater reuse ; {S}oil microcosm ; {M}icrobial communities ; {FRANCE}},
  booktitle = {},
  journal = {{S}cience of the {T}otal {E}nvironment},
  volume = {931},
  numero = {},
  pages = {172977 [11 ]},
  ISSN = {0048-9697},
  year = {2024},
  DOI = {10.1016/j.scitotenv.2024.172977},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090738},
}