@article{fdi:010090713,
  title = {{M}onocytes, particularly nonclassical ones, lose their opsonic and nonopsonic phagocytosis capacity during pediatric cerebral malaria},
  author = {{V}ianou, {B}. and {R}oyo, {J}ade and {D}echavanne, {S}. and {B}ertin, {G}wladys and {Y}essoufou, {A}. and {H}ouze, {S}. and {F}aucher, {J}. {F}. and {A}ubouy, {A}gn{\`e}s and {N}euro{CM} group,},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntroduction {I}nnate immunity is crucial to reducing parasite burden and contributing to survival in severe malaria. {M}onocytes are key actors in the innate response and, like macrophages, are plastic cells whose function and phenotype are regulated by the signals from the microenvironment. {I}n the context of cerebral malaria ({CM}), monocyte response constitutes an important issue to understand. {W}e previously demonstrated that decreased percentages of nonclassical monocytes were associated with death outcomes in {CM} children. {I}n the current study, we postulated that monocyte phagocytosis function is impacted by the severity of malaria infection.{M}ethods {T}o study this hypothesis, we compared the opsonic and nonopsonic phagocytosis capacity of circulant monocytes from {B}eninese children with uncomplicated malaria ({UM}) and {CM}. {F}or the {CM} group, samples were obtained at inclusion ({D}0) and 3 and 30 days after treatment ({D}3, {D}30). {T}he phagocytosis capacity of monocytes and their subsets was characterized by flow cytometry and transcriptional profiling by studying genes known for their functional implication in infected-red blood cell (i{RBC}) elimination or immune escape.{R}esults {O}ur results confirm our hypothesis and highlight the higher capacity of nonclassical monocytes to phagocyte i{RBC}. {W}e also confirm that a low number of nonclassical monocytes is associated with {CM} outcome when compared to {UM}, suggesting a mobilization of this subpopulation to the cerebral inflammatory site. {F}inally, our results suggest the implication of the inhibitory receptors {LILRB}1, {LILRB}2, and {T}im3 in phagocytosis control.{D}iscussion {T}aken together, these data provide a better understanding of the interplay between monocytes and malaria infection in the pathogenicity of {CM}.},
  keywords = {cerebral malaria ; monocyte ; nonclassical monocytes ; phagocytosis ; children ; {B}enin ; flow cytometry ; {BENIN}},
  booktitle = {},
  journal = {{F}rontiers in {I}mmunology},
  volume = {15},
  numero = {},
  pages = {1358853 [12 p.]},
  ISSN = {1664-3224},
  year = {2024},
  DOI = {10.3389/fimmu.2024.1358853},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090713},
}