Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria /Fraering, Jeremy Salnot, V. Gautier, E. F. Ezinmegnon, S. Argy, N. Peoc'h, K. Manceau, H. Alao, J. Guillonneau, F. /Migot Nabias, Florence /Bertin, Gwladys Kamaliddin, C. /Aubouy, Agnès Affolabi, D. Biokou, B. /Cot, Michel Degbelo, J. E. /Deloron, Philippe Dramane, L. Faucher, J. F. Guillochon, E. Houzé, L. /Guemouri, Sayeh Houze. S. Joste, V. Labrunie, A. Ladipo, Y. Lathiere, T. Massougbodji, A. Mowendabeka, A. Papin, J. Pipy, B. Preux, P. M. Raymondeau, M. Royo, J. Sossou, D. Techer, B. Vianou, B. Cerebral Malaria LC-MS/MS Plasmodium falciparum Biomarkers Red Blood Cells Cerebral malaria (CM), the most lethal complication of Plasmodium falciparum severe malaria (SM), remains fatal for 15-25% of affected children despite the availability of treatment. P. falciparum infects and multiplies in erythrocytes, contributing to anemia, parasite sequestration, and inflammation. An unbiased proteomic assessment of infected erythrocytes and plasma samples from 24 Beninese children was performed to study the complex mechanisms underlying CM. A significant down-regulation of proteins from the ubiquitin-proteasome pathway and an up-regulation of the erythroid precursor marker transferrin receptor protein 1 (TFRC) were associated with infected erythrocytes from CM patients. At the plasma level, the samples clustered according to clinical presentation. Significantly, increased levels of the 20S proteasome components were associated with SM. Targeted quantification assays confirmed these findings on a larger cohort (n = 340). These findings suggest that parasites causing CM preferentially infect reticulocytes or erythroblasts and alter their maturation. Importantly, the host plasma proteome serves as a specific signature of SM and presents a remarkable opportunity for developing innovative diagnostic and prognostic biomarkers. 2024 text https://www.documentation.ird.fr/hor/fdi:010090710 fdi:010090710 Fraering Jeremy, Salnot V., Gautier E. F., Ezinmegnon S., Argy N., Peoc'h K., Manceau H., Alao J., Guillonneau F., Migot Nabias Florence, Bertin Gwladys, Kamaliddin C., Aubouy Agnès, Affolabi D., Biokou B., Cot Michel, Degbelo J. E., Deloron Philippe, Dramane L., Faucher J. F., Guillochon E., Houzé L., Guemouri Sayeh, Houze. S., Joste V., Labrunie A., Ladipo Y., Lathiere T., Massougbodji A., Mowendabeka A., Papin J., Pipy B., Preux P. M., Raymondeau M., Royo J., Sossou D., Techer B., Vianou B.. Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria. 2024, 16 (2), 319-333 EN BENIN