@article{fdi:010090666,
  title = {{D}irofilaria sp. and blood meal analysis in mosquitoes collected in {V}ojvodina and {M}ačva, and the first report of {S}etaria tundra ({I}ssaitshikoff & {R}ajewskaya, 1928) in {S}erbia},
  author = {{S}iljegovic, {S}. and {M}ouillaud, {T}h{\'e}o and {J}iolle, {D}avy and {P}etric, {D}. and {I}gnjatovic-{C}upina, {A}. and {V}asic, {A}. and {P}aupy, {C}hristophe and {K}avran, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{D}irofilaria immitis and {D}. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. {W}hile {D}. immitis causes cardiopulmonary dirofilariasis, {D}. repens causes subcutaneous infections in dogs and other carnivores. {D}espite the extensive knowledge on these parasites, little is known about their natural vectors in {S}erbia. {T}he parasite {S}etaria tundra, known to infect deer, has not yet been detected in {S}erbia but has been documented in neighboring countries. {T}hus, the aim of this study was to (i) further map out {D}irofilaria sp. hotspots in the {V}ojvodina {P}rovince and detect {S}. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in {S}erbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. {A} total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. {M}olecular biology methods, based on conventional {PCR}, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. {W}hen the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for {S}anger sequencing, further confirming the presence of nematodes and species assignation. {D}. immitis was detected in three {C}ulex pipiens mosquitoes collected in {Z}renjanin ({A}ugust 2021) and {G}logonj and {S}vetozar {M}ileti & cacute; (both in {J}uly 2021). {A}dditionally, {S}etaria tundra was detected in {A}edes vexans collected in {I} & dstrok;o & scaron; (mid-{A}ugust 2021) and {A}edes caspius, which was collected in {M}ali {I} & dstrok;o & scaron; (end of {J}uly 2021). {T}his work identifies two new locations where {D}. immitis occurs in {V}ojvodina, and is the first report of {S}. tundra in {S}erbian territory. {B}lood-meal analysis provided insights into the preferences of mosquitoes that were positive for {D}irofilaria sp. and {S}. tundra.},
  keywords = {{D}irofilaria sp. ; {S}etaria tundra ; mosquito surveillance ; {C}ox1 gene ; {SERBIE}},
  booktitle = {},
  journal = {{A}nimals},
  volume = {14},
  numero = {9},
  pages = {1255 [15 p.]},
  ISSN = {2076-2615},
  year = {2024},
  DOI = {10.3390/ani14091255},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090666},
}