@article{fdi:010090640,
  title = {{A} simple, field-applicable method to increase the infectivity of wild isolates of {P}lasmodium falciparum to mosquito vectors},
  author = {{O}uattara, {S}. {B}. and {H}ien, {D}fds and {N}ao, {E}. {T}. and {P}ar{\'e}, {P}. {S}. {L}. and {G}uissou, {E}. and {C}ohuet, {A}nna and {M}orlais, {I}sabelle and {Y}erbanga, {R}. {S}. and {D}abir{\'e}, {K}. {R}. and {O}u{\'e}draogo, {J}. {B}. and {M}ouline, {K}arine and {L}ef{\`e}vre, {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he direct membrane feeding assay ({DMFA}), whereby gametocyte-infected blood is collected from human donors and from which mosquitoes feed through a membrane, is proving essential for assessing parameters influencing {P}lasmodium transmission potential in endemic countries. {T}he success of {DMFA}s is closely tied to gametocyte density in the blood, with relatively high gametocytaemia ensuring optimal infection levels in mosquitoes. {A}s transmission intensity declines with control efforts, the occurrence of asymptomatic individuals with low gametocyte densities, who can significantly contribute to the infectious reservoir, is increasing. {T}his poses a limitation to studies relying on the experimental infection of large numbers of mosquitoes with natural isolates of {P}lasmodium. {A} simple, field-applicable method is presented for improving parasite infectivity by concentrating {P}lasmodium falciparum gametocytes. {M}ethods {A}nopheles gambiae received one of the following 5 blood treatments through {DMFA}: (i) whole blood ({WB}) samples from naturally-infected donors; (ii) donor blood whose plasma was replaced with the same volume of {P}lasmodium-naive {AB} + serum (1:1 control); (iii) plasma replaced with a volume of malaria-na & iuml;ve {AB} + serum equivalent to half (1:1/2), or to a quarter (1:1/4), of the initial plasma volume; and (v) donor blood whose plasma was fully removed ({RBC}). {T}he experiment was repeated 4 times using 4 distinct wild parasite isolates. {S}even days post-infection, a total of 1,095 midguts were examined for oocyst presence. {R}esults {S}ubstituting plasma with reduced amounts (1:1/2 and 1:1/4) of {P}lasmodium-naive {AB} + serum led to a 31% and 17% increase of the mosquito infection rate and to a 85% and 308% increase in infection intensity compared to the 1:1 control, respectively. {T}he full removal of plasma ({RBC}) reduced the infection rate by 58% and the intensity by 64% compared to the 1:1 control. {R}educing serum volumes (1:1/2; 1:1/4 and {RBC}) had no impact on mosquito feeding rate and survival when compared to the 1:1 control. {C}onclusions {C}oncentrating gametocytic blood by replacing natural plasma by lower amount of naive serum can enhance the success of mosquito infection. {I}n an area with low gametocyte density, this simple and practical method of parasite concentration can facilitate studies on human-to-mosquito transmission such as the evaluation of transmission-blocking interventions.},
  keywords = {{P}lasmodium falciparum ; {A}nopheles gambiae ; {DMFA} ; {E}xperimental infections ; {T}ransmission-blocking interventions ; {G}ametocyte infectivity ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {23},
  numero = {1},
  pages = {135 [9 p.]},
  year = {2024},
  DOI = {10.1186/s12936-024-04969-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090640},
}