@article{fdi:010090612, title = {{T}ranscriptome analysis reveals a diverse range of novel viruses in {A}ustralian sugarcane soldier fly ({I}nopus flavus) larvae}, author = {{D}ivekar, {G}. and {C}olmant, {A}gathe and {F}urlong, {M}. {J}. and {E}tebari, {K}.}, editor = {}, language = {{ENG}}, abstract = {{I}n {A}ustralia, {S}oldier flies ({I}nopus spp.) are economically significant pests of sugarcane that currently lack a viable management strategy. {D}espite various research efforts, the mechanisms underlying the damage caused by soldier fly larvae remain poorly understood. {O}ur study aims to explore whether this damage is associated with the transmission of plant viruses during larval feeding. {W}e also explore the larval transcriptome to identify any entomopathogenic viruses with the potential to be used as biocontrol agents in future pest management programs. {S}even novel virus sequences are identified and characterised using de novo assembly of {RNA}-{S}eq data obtained from salivary glands of larvae. {T}he novel virus sequences belong to different virus families and are tentatively named {SF}-associated anphevirus ({SF}a{AV}), {SF}-associated orthomyxo-like virus ({SF}a{OV}), {SF}-associated narna-like virus ({SF}a{NV}), {SF}-associated partiti-like virus ({SF}a{PV}), {SF}-associated toti-like virus ({SF}a{TV}-1 and {SF}a{TV}-2) and {SF}-associated densovirus ({SF}a{DV}). {T}hese newly identified viruses are more likely insect-associated viruses, as phylogenetic analyses show that they cluster with other insect-specific viruses. {S}mall {RNA} analysis indicates prominent peaks at both 21 nt and 26-29 nt, suggesting the activation of host si{RNA} and piwi{RNA} pathways. {O}ur study helps to improve understanding of the virome of soldier flies and could identify insect viruses for deployment in novel pest management strategies.}, keywords = {soldier fly virome ; transcriptome analysis ; next-generation sequencing ; insect-specific viruses ; {AUSTRALIE}}, booktitle = {}, journal = {{V}iruses}, volume = {16}, numero = {4}, pages = {516 [19 p.]}, year = {2024}, DOI = {10.3390/v16040516}, URL = {https://www.documentation.ird.fr/hor/fdi:010090612}, }