%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cortez, M. V.
%A Scotta, A. V.
%A Miranda, Agustin Ramiro
%A Soria, E. A.
%T Polyphenol analysis in human milk by a rapid, costeffective, and accurate micromethod : translational development
%D 2024
%L fdi:010090611
%G ENG
%J Revista Paulista de Pediatria
%@ 0103-0582
%K Analytic sample preparation methods ; Clinical laboratory techniques ; Milk ; human ; Polyphenols ; Technology assessment ; biomedical
%K ARGENTINE
%M ISI:001208853500016
%P e2023186 [9 ]
%R 10.1590/1984-0462/2024/42/2023186
%U https://www.documentation.ird.fr/hor/fdi:010090611
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-06/010090611.pdf
%V 42
%W Horizon (IRD)
%X Objective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes. Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p<0.05). The best technique was applied to 284 HM samples to determine their polyphenolic content and its association with maternal diet by multifactorial linear regression. Results: Acidic extraction successfully recovered the gallic acid reference standard, whereas alkaline extraction overestimated it. Calibration curves for all methods were linear (R-2>0.96) up to 500 mg/L. All bicarbonate-based Folin-Ciocalteu methods assayed were stable and repeatable, whereas Fast Blue BB-based variants were not. HM polyphenols (mean=94.68 mg/L) positively correlated to the dietary intake of hydroxycinnamic acids, the most consumed polyphenolic family in this population. Conclusions: A bicarbonate-based Folin-Ciocalteu micromethod allowed the accurate determination of polyphenols in HM, which might be useful for translational research settings and HM banks.
%$ 054