@article{fdi:010090611,
  title = {{P}olyphenol analysis in human milk by a rapid, costeffective, and accurate micromethod : translational development},
  author = {{C}ortez, {M}. {V}. and {S}cotta, {A}. {V}. and {M}iranda, {A}gustin {R}amiro and {S}oria, {E}. {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {T}o develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk ({HM}) volumes. {M}ethods: {A}cid and alkaline extractions were compared using two dyes: {F}olin-{C}iocalteu and {F}ast {B}lue {BB}. {L}inearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 {HM} samples and compared using the {K}ruskal-{W}allis {H} test (p<0.05). {T}he best technique was applied to 284 {HM} samples to determine their polyphenolic content and its association with maternal diet by multifactorial linear regression. {R}esults: {A}cidic extraction successfully recovered the gallic acid reference standard, whereas alkaline extraction overestimated it. {C}alibration curves for all methods were linear ({R}-2>0.96) up to 500 mg/{L}. {A}ll bicarbonate-based {F}olin-{C}iocalteu methods assayed were stable and repeatable, whereas {F}ast {B}lue {BB}-based variants were not. {HM} polyphenols (mean=94.68 mg/{L}) positively correlated to the dietary intake of hydroxycinnamic acids, the most consumed polyphenolic family in this population. {C}onclusions: {A} bicarbonate-based {F}olin-{C}iocalteu micromethod allowed the accurate determination of polyphenols in {HM}, which might be useful for translational research settings and {HM} banks.},
  keywords = {{A}nalytic sample preparation methods ; {C}linical laboratory techniques ; {M}ilk ; human ; {P}olyphenols ; {T}echnology assessment ; biomedical ; {ARGENTINE}},
  booktitle = {},
  journal = {{R}evista {P}aulista de {P}ediatria},
  volume = {42},
  numero = {},
  pages = {e2023186 [9 ]},
  ISSN = {0103-0582},
  year = {2024},
  DOI = {10.1590/1984-0462/2024/42/2023186},
  URL = {https://www.documentation.ird.fr/hor/fdi:010090611},
}